BLASTP 2.2.21+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Stephen
F. Altschul, John C. Wootton, E. Michael Gertz, Richa
Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and
Yi-Kuo Yu (2005) "Protein database searches using
compositionally adjusted substitution matrices", FEBS J.
272:5101-5109.


RID: 3H99D5WA013


Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
           9,090,450 sequences; 3,112,979,771 total letters
Query= gi|168481420|gb|ACA24900.1| WfgL [Escherichia coli]
Length=257


                                                                   Score     E
Sequences producing significant alignments:                       (Bits)  Value

gb|ACA24900.1|  WfgL [Escherichia coli]                             526    9e-150
ref|YP_001744314.1|  glycosyl transferase family protein [Esch...   122    3e-28 

ALIGNMENTS
>gb|ACA24900.1| WfgL [Escherichia coli]
Length=257

 Score =  526 bits (1355),  Expect = 9e-150, Method: Compositional matrix adjust.
 Identities = 257/257 (100%), Positives = 257/257 (100%), Gaps = 0/257 (0%)

Query  1    MRLMANIYIATHKNYPFPPGYIPLHVGKRLSSVYVPNAIGDDSKNNISDLNPFFCELTGL  60
            MRLMANIYIATHKNYPFPPGYIPLHVGKRLSSVYVPNAIGDDSKNNISDLNPFFCELTGL
Sbjct  1    MRLMANIYIATHKNYPFPPGYIPLHVGKRLSSVYVPNAIGDDSKNNISDLNPFFCELTGL  60

Query  61   YWIWQNDADDVIGLVHYRRYFKHKNDYITIKNKKIASCNDLIKEFDSYDLILPKPSYLFK  120
            YWIWQNDADDVIGLVHYRRYFKHKNDYITIKNKKIASCNDLIKEFDSYDLILPKPSYLFK
Sbjct  61   YWIWQNDADDVIGLVHYRRYFKHKNDYITIKNKKIASCNDLIKEFDSYDLILPKPSYLFK  120

Query  121  KTLKEQYIKYHHEDDLIKLRQIIEKKYPDYISTFDTVLNGNKGYYCNMFIAKKNIIEPYF  180
            KTLKEQYIKYHHEDDLIKLRQIIEKKYPDYISTFDTVLNGNKGYYCNMFIAKKNIIEPYF
Sbjct  121  KTLKEQYIKYHHEDDLIKLRQIIEKKYPDYISTFDTVLNGNKGYYCNMFIAKKNIIEPYF  180

Query  181  QWVFDILFELKSSLDISGYDDYQKRVFGFLSERLFAVWIEYNKNRIQITHRSVVEIESNK  240
            QWVFDILFELKSSLDISGYDDYQKRVFGFLSERLFAVWIEYNKNRIQITHRSVVEIESNK
Sbjct  181  QWVFDILFELKSSLDISGYDDYQKRVFGFLSERLFAVWIEYNKNRIQITHRSVVEIESNK  240

Query  241  VISVKRYIRNFLAKLTG  257
            VISVKRYIRNFLAKLTG
Sbjct  241  VISVKRYIRNFLAKLTG  257


>ref|YP_001744314.1| glycosyl transferase family protein [Escherichia coli SMS-3-5]
 gb|ACB15494.1| glycosyl transferase family 8 [Escherichia coli SMS-3-5]
Length=630

 Score =  122 bits (307),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 86/281 (30%), Positives = 133/281 (47%), Gaps = 53/281 (18%)

Query  7    IYIATHKNYPFPPGYI--PLHVGKRLSSVYVP-NAIGDDSKNNISDLNPFFCELTGLYWI  63
            IY   HK   F    I  PLHVGK  ++ Y      GDDS +NIS  NPF+CELT  YW+
Sbjct  6    IYTCHHKPSAFLNASIIKPLHVGK--ANTYNDIGCEGDDSGDNISFKNPFYCELTAHYWV  63

Query  64   WQNDA-DDVIGLVHYRRYFK------HKND----------------YITIKNKKIASCND  100
            W+N++  D +G +HYRR+        H  D                   + ++ I++C  
Sbjct  64   WKNESLADYVGFMHYRRHLNFAEQQNHPEDNWGVVNYPLINAEYESQFGLSDESISTC--  121

Query  101  LIKEFDSYDLILPKPSYLFKKTLK---EQYIK--YHHEDDLIKLRQIIEKKYPDYISTFD  155
                 D YDL+LPK   +     K   + Y K  + H  D      ++E+ YP Y +   
Sbjct  122  ----VDGYDLLLPKKWSVTSAGSKNNLDHYAKGEFLHIKDYQSALDVVEELYPQYKAAIQ  177

Query  156  TVLNGNKGYYCNMFIAKKNIIEPYFQWVFDILFELKSSLDISGYDDYQKRVFGFLSERLF  215
               N   GYY NMF+ +K++   Y +W+F IL  L+  + ++ Y+  +KRV G ++ERLF
Sbjct  178  QFNNATDGYYTNMFVMRKDMFLDYSEWLFAILSNLEDRISMNNYNAQEKRVIGHIAERLF  237

Query  216  AVWIEYNKNRIQITHRSVVEIESNKVISVKRYIRNFLAKLT  256
             ++I              ++ + +K + +K   R F+   T
Sbjct  238  NIYI--------------IKCQQDKQLKIKELQRTFVTAET  264



  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Jun 16, 2009  5:41 PM
  Number of letters in database: 26,573,871
  Number of sequences in database:  84,272

Lambda     K      H
   0.324    0.142    0.438 
Gapped
Lambda     K      H
   0.267   0.0410    0.140 
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 84272
Number of Hits to DB: 3574057
Number of extensions: 151138
Number of successful extensions: 249
Number of sequences better than 0.1: 0
Number of HSP's better than 0.1 without gapping: 0
Number of HSP's gapped: 250
Number of HSP's successfully gapped: 0
Length of query: 257
Length of database: 26573871
Length adjustment: 102
Effective length of query: 155
Effective length of database: 17978127
Effective search space: 2786609685
Effective search space used: 2786609685
T: 11
A: 40
X1: 15 (7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 79 (35.0 bits)