Bioorganic Chemistry

Serogroup

O112ab

Serotype

Strain

ECODAB#

111

Structure

bDGlcNAc(1->3)
            |
   ->4)aDGalNAc(1->4)aDGlc(1->4)aLIdoA(1->3)bDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

4.55 3.82 3.55 3.44 3.45 3.79 3.94
5.30 4.20 4.00 4.45 4.06 3.78 3,81
5.20 3.50 3.83 3.62 3.75 3.70 3.81
5.04 3.63 3.99 4.15 4.75
4.97 4.14 3.86 4.00 3.67 3.75 3.77

Conditions

Solvent: D2O; Temp.: 35C

Reference

acetone=2.225ppm

Comment

[1]

¹³C-NMR

104.1 57.2 75.2 71.8 77.4 62.5
99.9 50.2 77.7 75.8 72.3 61.8
97.6 73.1 75.2 76.7 72.3 62.2
104.9 70.9 69.7 76.0 69.7 175.0
102.4 52.7 81.0 69.7 76.7 62.6

Conditions

Solvent: D2O; Temp.: 35C

Reference

acetone=31.45ppm

Comment

[1]

wzx (flippase)

TrEMBL entry

B5L401

Sequence

MNLLKGTIIYTLSNLTIKFGAVLLLPILTHLLNPEEYGMVGLYVTLTSFLTIILGLGFYTPLMKTQSEQK SNIATSSHVNFCAIIFLIAVYLLLLLFLYFILKSSLVIDLLGEVKLDSKLIYLAVIVSMFSAINIIMNTS FRMDENYILVAILSILSFVLFYSSAIIFIKQFNYGGLGYIYGNLLSSIIIFFISFTCYYRKLSIAFSWHN VKFLCGNGIPMVFVELSDKIIEASDRFILVRYISLSSLGVYTLALTGCKVLNVVFNSYISAILPSIYKSV ESREDSNYIKVKLENAYVLVVMMVFLGQLISKEIIDIIFPASYSNLFFVFILALPAISLQYYYFLDFYFH RSEDSRFILCFTIATSIINIILNLIFVPKYGVYASLLSTYISYLIRTLVEIKFIARRYKLKFSVLKVLMG SFVLYTVPLLYHYGYYTVLLRVTLSIVLFIGIIMFYKKSKIFN

wzy (polymerase)

TrEMBL entry

B5L402

Sequence

MVNAKNFVLIIFDVICLFLNVFVYVPRLIIGVLTVDKTLIITFPLMLSVLAYNFIPPETYDLYRHYENYQ NFLNNNEVSFIRDFFLYFLFTVGRLFDLNNGFIAFVSCYILYFYWLKILYINFDYKANKKNFPLYFFIFF ITPPLVIYTGVRFSTGLILTLYGIVNWIQLRNVKLGIVYIICGLTAHFSMALIALIFIFTSYFNNIFSNK YLRVGLVFSALLVISFNEQLVRFLTTVIEFINQLFDWNFISIDTYITGKWGLERAQELNSTGQLVLGIRN YGMLFLILLFCPLISFNKNKYTNFYYVMCAVTILVFPFTAVFDRYGTFLIFFIVYYLSTLQKKTNIEIIY FLMLIVYLIFFRTTDIKDNISVYTESYSNILNLSLIKVLLGI

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfeF	1	4	bDGlcNAc(1->	->3)aDGalNAc	O28ac WbeX (ECODAB) O42 WbeX (ECODAB) O66 WfaC (ECODAB) O113 WbnA (ECODAB) O124 WfeS (ECODAB) O112ac WfeM (ECODAB)	ACD37087	[2]
WfeG	2	3	aDGalNAc(1->	->4)aDGlc	O104 WbwB (ECODAB) O22 WbhP (ECODAB) O111 WbdH (ECODAB) O167 WfdI (ECODAB)	ACD37088	[2]
WfeH	3	2	aDGlc(1->	->4)aLIdoA		ACD37090	[2]
WfeI	4	1	aLIdoA(1->	->3)bDGalNAc	O143 WfdV (ECODAB) O21 WclQ (ECODAB) O157:H7 WbdN (ECODAB) O24 WbdN (ECODAB) O130 WffJ (ECODAB)	ACD37091	[2]

References

[1] Carbohydr. Res. 343 (2008) 571-575 [PubMed 18062946]

[2] FEMS Microbiol Rev. 32 (2008) 627-653 [PubMed 18422615]

Edit this entry

Wed May 22 21:24:55 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group