Bioorganic Chemistry

Serogroup

O112ac

Serotype

Strain

ECODAB#

112

Structure

   (S)-pyr
    |
    4,6
bDGlcNAc(1->3)-(3Ac(c.70%)
            |
    ->4)aDGalNAc(1->4)aDGlc(1->4)bDGal(1->3)bDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Shigella D2. E.coli lacks O-acetyl groups [2]

Cross-reacts with

¹H-NMR

4.55 3.80 3.55 3.42 3.43 3.78 3.93
5.34 4.20 3.97 4.45 4.05 3.72 3.80
4.89 3.51 3.92 3.64 4.20 3.68 3.88
4.59 3.56 3.73 4.01 3.68
4.96 4.14 3.88 4.14 3.74

Conditions

Solvent: D2O; Temp.: 55C

Reference

acetone=2.225ppm

Comment

[1]

¹³C-NMR

104.1 57.2 75.1 71.6 77.3 62.5
99.7 50.1 77.5 75.7 72.7 61.5
101.4 73.4 74.9 76.8 72.0 62.2
106.5 72.1 73.5 79.0 76.4
102.4 52.5 82.1 69.8 76.5

Conditions

Solvent: D2O; Temp.: 55C

Reference

acetone=31.45ppm

Comment

[1]

wzx (flippase)

TrEMBL entry

B5L3T4

Sequence

MSMLLNLIIPIISFGIVSIMGLIMTPFFINNFGYEGLGLISNSNALIQSLFFAGGAFTTVYSREYSNAVN NIEKSVLLRELNNILTAIFFIAVLLSIVFYIFYIFKDNFSYNYYALVFLASAIIIISQSFNVFPFAANRL YINSVIDCARTLLRNILCMALFYLSIDNVLSNGWAMILSAVITLLVLKLVFKENKVFYVGRMSNNYNIKN SFWVCINQGGAYIFSFADVFLINLMLGHYIGGSYAVLIQIPVLLKSVSVVAIGSISSFTVKVHQRFEKDR TIIKKIINKVVVFFSFFISLAFILVDLYKESFFSIWLPTAVTSSMLKQFNYICIIMMLLSISSLVNIFLV AWGHFIIPAKATVFSAVVSVLVVVLLYFFNYKLSEMIIFSVIYLMLILKNLICYLYFFMQSGLSLIHFIF SLILSFCVITINFLVAKSIRYYIESDFLSIVILFFVMMTISLPLLWDFKKLREYI

wzy (polymerase)

TrEMBL entry

B5L3T0

Sequence

MIKKNNIYRFYILVCVFSGFYIFGFFPAPYYLCLILGFVFLSLSKIKHVDKNILFLLCIVIIYMVFMLFN SRINVWSYYFIGLLTYFIIILLRNNLDYYQKIAIVRKTVLISLLLVILDTIYRFYYPREEYLLAIEQQGK IDYLFYGYKHSFLFQDSNFVGLYILSVFFLFRDNMSIFKWKKSILLTIIILIILSLSRAAIVALILTECI RMLCGMKVDVRLKIVSLIFMAIPTIYLLFVLVANIDDTSFESKFMLLNKFLTTFQERNFTELLFGWGLDN TREHWGIAAHSLFNTLLLEMGLVGFIIITISIVYCWCLNTKTLYHFMGLSIAFLSFGLIFTPFFIPLGLN IIMRDVSNEKL

Comment

From [3]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfeJ	1	1	bDGal(1->	->3)bDGalNAc	O174 WbcZ (ECODAB) O146 WbwW (ECODAB) O155 WclG (ECODAB) O104 WbwC (ECODAB) O107 WclZ (ECODAB) O117 WbeD (ECODAB)	187880493
WfeK	2	2	aDGlc(1->	->4)bDGal	O117 WbeC (ECODAB) O117 WclY (ECODAB)	187880494
WfeL	3	3	aDGalNAc(1->	->4)aDGlc	O22 WbhT (ECODAB) O104 WbwB (ECODAB) O168 WfeW (ECODAB) O130 WffI (ECODAB)	187880496
WfeM	4	4	bDGlcNAc(1->	->3)aDGalNAc	O124 WfeS (ECODAB) O3 WclS (ECODAB) O112ab WfeF (ECODAB) O66 WfaC (ECODAB)	ACD37016

References

Edit this entry

Mon May 20 06:12:17 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group