Bioorganic Chemistry

Serogroup

O168

Serotype

Strain

ECODAB#

114

Structure

aLFuc(1->3)(2/3/4-Ac totally c.50%)  
          |
   ->4)aDGlcNAc(1->4)aDGlcA(1->3)aLFuc(1->3)bDGlcNAc(1->
          6
          |
          Ac(c.50%)

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Shigella D4. E.coli O168 lacks O-acetyl groups from the Fuc residue

Cross-reacts with

¹H-NMR

5.11 3.68 3.95 3.79 4.72 1.25
5.17 4.13 3.91 3.87 3.72 3.71 3.83
5.17 3.57 3.73 3.73 4.18	
4.97 3.88 3.89 3.91 4.31 1.14
4.55 3.80 3.63 3.31 3.44 3.59 3.96

Conditions

Solvent: D2O; Temp.: 30C

Reference

TSP=0.0ppm

Comment

[1]

¹³C-NMR

99.7 69.2 70.7 73.3 68.0 16.9
99.6 55.3 73.3 79.3 73.3 60.8
102.0 73.3  74.6 79.3 73.7 175.7
101.6 68.7 74.6	 73.7 68.4 16.5
101.5 57.0 81.9	 77.6 63.2

Conditions

Solvent: D2O; Temp.: 30C

Reference

acetone=31.45ppm

Comment

[1]

wzx (flippase)

TrEMBL entry

B5L3Q8

Sequence

MFRQINFKNLEIMKQILISFSFRLMGPVIMLLTTPQLLSTLGNVEYGVWSLLITTISWLTICDFGISNQI RNESKILIVQDNKESIREIYTAAVIISFFITLLCCVIISFINIDLIYKLFLSCVFLFAIETNICRALCLS LNKPELASAVTNIPNLFLLIYVFCYSSFNINNVMDIYCYGSAMMFVSIFIILYKFNIKLFWPVFHPSYLR FSIMKYSLLRGGGGFFMVQLVSMGLIISDRYIISSIFGVEDVTKYDLIFKVSNFCIMFFSIVNMVLWSKF TEFWMQGFYTKIKVIFKVFDLCFLLLVIVLILILNHIHWIFELWLNVTNENYNEVYITGIVLYLVGYYWM SAYSSFLNGISIIKKQFIIQGGGLLIKAITLLIAMNILKVFKVEYIILLGGACLFIVGFLFRKCAHDKIN SCI

wzy (polymerase)

TrEMBL entry

B5L3R2

Sequence

MNCKFNIWDIQLGLILISVLSYAFLNLGFLPLNISHLIILFVLITLSVNLLISQSIKITGLFLTLFVSFI LLSAYSLLKYYDVQKVKNIINFFIFFSVAAIVINNCSADKIIKFYYKLTKIFIFFALLQWVLYYLNIGTL YTYSFLGLKEVNISTSGYLIRLFSIASEPAALCGILLPAIYLSINRIVNKGKEVTLSYSVIVLFVILNTF SLVGYIYIIICLIVALYVGNKISLSKIFIFTICLALLVFILFQSDSIQQRLNEITSLDKMASSDNLSVIA IYSNLQIALISLSDNLIFGGGIFSHPYTYDHYISQLYAGGGPRMELNKDDAASLYIRALSETGILGFCIL NGLIIYLMKRCDKSKINYPYNIAFTIAFALLGIRAGSVNYIIIWFYFFSAIRFVNEGRLK

Comment

From [4]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfeV	1				O159 WfgS (ECODAB) O168 WfeY (ECODAB) O157 WbdO (ECODAB)	187880473	[4]
WfeW	2				O103 WbtF (ECODAB) O22 WbhT (ECODAB) O32 WfeE (ECODAB) O112ac WfeL (ECODAB)	187880474	[4]
WfeX	3				O56 WfaQ (ECODAB) GlcNAc transferase [Escherichia coli] O24 WfaO (ECODAB) O29 WffZ (ECODAB)	187880476	[4]
WfeY	4	3	aDGlcNAc(1->	->4)aDGlcA	O159 WfgS (ECODAB) O168 WfeV (ECODAB)	187880481	[4]

References

[1] Carbohydr Res.17 (2007) 2676-2681 [PubMed 17880932]

[2] Bioorg Khim.3 (1977) 1226–1233

[3] Bioorg Khim (2008) 513-521. [PubMed 18695724]

[4] FEMS Microbiol.(2008) 627-653 [PubMed 18422615]

Edit this entry

Mon May 20 23:57:45 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group