Bioorganic Chemistry

Serogroup

O40

Serotype

Strain

ECODAB#

117

Structure

->2)bDGal(1->4)bDMan(1->4)aDGal(1->3)bDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Shigella D9

Cross-reacts with

¹H-NMR

4.47 3.72 3.72 3.87 3.71 3.78 3.78	
4.84 4.20 3.76 3.74 3.52 3.85 4.16	
5.40 3.88 3.89	4.22	3.90 3.68  3.84
4.88 3.81 3.74 3.69 3.42 3.74 3.91 2.06

Conditions

Solvent: D2O; Temp.: 30C

Reference

TSP=0.0ppm

Comment

[1]

¹³C-NMR

103.2 78.9 75.2 70.4 76.7 62.4
102.2 71.3 73.0 79.2 76.5 62.2
100.8 70.0 70.8 77.6 71.8 61.5
102.6 56.1 81.5 72.3 76.9 62.3 23.9 175.5

Conditions

Solvent: D2O; Temp.: 30C

Reference

acetone=31.45ppm

Comment

[1]

wzx (flippase)

TrEMBL entry

B5L435

Sequence

MFQNGVYISFYSFIAKATVFLCGPLTLLLISHKLSTEEIAFYYTFFNLIALQQLVELGLGFTLKQCISHA YKIENDVWLYDSKKIVKSYIQFGLYWFIGVSCIIIFVVGPLGLIFYGDYEGSIKWQGAWLGTVGVLAFSI ILVPIQIAIEATQNQIANISLSSYIFSHNSLSLWVFIYNDFKLYSIPLSMLIATYFLLIALKNHYIQLYN KLAFVKEMDKFWTIFSKLWPLFSRVAIVWGVGFLLWNGFNLISFKIFSPEEAGRIIFSITLARAGFTIAE SIISAQTTVVSNMISKGENTEAAKYYRKYQLISFAVLIIGYGFYYVVKCFFSDFYLFDKLMPNSFITSVF VFFVLLLILTTNSNFIRCYKIEPFVKVSVWHSLGVPMSFLLSSLYFPMYLYPCSFVLFISIIWSQLISKR ILSKIG

wzy (polymerase)

TrEMBL entry

B5L433

Sequence

MSHFGGGLMKSVNKFNFYLAIFIGMFDLTIFSTGNSLGQALTTVLLLFSILSDINKVFLKPFFFVISYIV LALLVSLFHGETSYHYSFIIVKTGLILFNAMVISQILILYADKKQISNFVNFIFLIQTISLLLYSNELFQ MMRGFFLSARSEHLYENFGDSYWYRDATISNQGYSGYALGALPLVIIAVHNIMKSKFFSIRYFVAVVCCG LGVSIAMLNGRSVFLFIPLYMIYLMLRPKEICLIYKIIPVILIIVSAIIIALTNLIYPSNFVLAFFEQKG KSYGIDSVDDLMYNHTILHFDITLFGLGKYLLSDGSAFVRSDIGYYRQMGVGGIFLVLFYIFSIWLAVRY VIYRDLSLFILFIILLVNFKQEAFNNAMLMYGAFLLAAYWVARDKIEITKK

Comment

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WffO	1	1	aDGal(1->	->3)bDGlcNAc	O3 WclR (ECODAB) O113 WbnE (ECODAB) O128 WbsH (ECODAB) O55:H7 WbgM (ECODAB) O87 WfgO (ECODAB) O148 WbbG (ECODAB)	187880608	[2]
WffP	2	3	bDGal(1->	->4)bDMan	O172 Orf3 (ECODAB) putative beta1,4-galactosyltransferase WaaX [Escherichia coli] O21 WclP (ECODAB)	ACD37116	[2]
WffQ	3	2	bDMan(1->	->4)aDGal	O56 WfaQ (ECODAB) O98 WbwV (ECODAB) O141 WfcD (ECODAB) O24 WfaO (ECODAB)	ACD37118	[2]

References

[1] Carbohydr Res. 342 (2007) 1275-1279 [PubMed 17395169]

[2] FEMS Microbiol Rev. 32 (2008)627-653. [PubMed 18422615]

Edit this entry

Sun May 19 00:18:23 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group