Bioorganic Chemistry

Serogroup

O87

Serotype

Strain

ECODAB#

119

Structure

   ->6)bDGal(1->3)bDGalNAc(1->4)aDGalNAc(1->3)bDGalNAc(1->
          |                                                    
 aLFuc(1->2)

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Shigella boydii 2

Cross-reacts with

¹H-NMR

5.22 3.76 3.64 3.72 4.22 1.21
5.04 4.07 3.89 4.13 3.80 3.71 3.87
4.60 3.62 3.80 3.88 3.76 3.87 3.93
4.54 4.02 3.76 4.07 3.62 3.78
4.51 3.99 3.91 4.02 3.69 3.78

Conditions

Solvent: D2O; Temp.: 70C

Reference

TSP=0.00ppm

Comment

[1]

¹³C-NMR

100.1 68.8 70.3 72.7 67.6 16.4
94.8 50.4 68.4 75.8 71.3 61.0
102.9 76.8 74.4 69.5 73.5 68.8
102.2 51.5 76.1 64.7 76.0 62.0
104.2 52.2 77.3 69.4 75.6 62.0

Conditions

Solvent: D2O; Temp.: 70C

Reference

dioxan=67.40ppm

Comment

[1]

wzx (flippase)

TrEMBL entry

B5L3M8

Sequence

MRVLGPEFFGVLGFCQASMQYLVLITDYGFNWTATQQIAKHRDNKLKINEIFWSVIFAKIFLASISFILL FVICWNVPKYHDVWYVLLSFSPFLLGNVIYPIWFFQGMEKMKAITVCTISARFLLIPLTFLLVHNSDDIW IAAIIQSSVNLLAGILSCCLIFKYRWISGFEFNLIKVKESICEGWHVFISTSAISLYTTSTTVILGFVAG NTAVGYFNAANTVRNAAQGLLNPVTQAIYPRVSALYEIDYVKALNLIKKMLRYIGGIAFLGSVILFFLAP VIIKYGLGDSYNNAVSILRWMAFLPFIILLSNIFGVQTMLTHGYKKQFSNILTVSGVLNLCIIYPLVCLY SADGAAIAMLITEIFVSFTMFCFLKNKKINIFK

wzy (polymerase)

TrEMBL entry

B5L3N1

Sequence

MSYIISVSTLIVILFFERNRMILPLSAVIISGVVVFICLSGNYYNGYDWINYLDNFQCLKYLGVYCWDKY DISYTLIVYLCSKIFDDYHSVVVVISVINTYCLCFFAYKNCRNPLLFITIYFSLYAWVLYSETLRQSLAV SFVFLSLSAFNKKHFVKSLALLGVASTFHISALFFLVLYIIKYKTNLTYKLFVLSFLIGAVFLPYIAASF FYLNPRILSYFLDTNWNEAQNLSIIQVSTTLFSLLLLFMVWRRLYNNNMLITKDEWGKANLIMFSLFSVL FALGKLHAQYFEVLSRVNYYIAIPAFVYLSNRIEDLRVTNKIIIYPFLMVYLLYSPVRFFYSLQKFDEYN NFYVDKLLLNKKINIEYLKNTRCSYLGNKFSACK

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfgL	1	4	aLFuc(1->	->2)bDGal		ACA24900	[2]
WfgN	2	3	bDGal(1->	->3)bDGalNAc	O22 WbhP (ECODAB) O56 WfaQ (ECODAB) O87 WfgO (ECODAB) O113 WbnD (ECODAB) O3 WclV (ECODAB) O152 WfgE (ECODAB)	ACA24903	[2]
WfgO	3	1	aDGalNAc(1->	->3)bDGalNAc	O22 WbhP (ECODAB) O55:H7 WbgM (ECODAB) O128 WbsH (ECODAB) O113 WbnE (ECODAB) O3 WclR (ECODAB) O40 WffO (ECODAB) O127 WbiN (ECODAB)	ACA24904	[2]

References

[1] Carbohydr. Res.343 (2008)695-742 [PubMed 18237721]

[2] FEMS Microbiol.(2008) 627-653 [PubMed 18422615]

Edit this entry

Fri May 24 14:09:52 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group