Bioorganic Chemistry

Serogroup

O107

Serotype

Strain

ECODAB#

138

Structure

->4)bDGalNAc(1->3)aLRha(1->4)aDGlcNAc(1->4)bDGal(1->3)aDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Identical to

Cross-reacts with

¹H-NMR

4.69,4.01,3.83,4.02,3.74,3.70
4.90,4.18,3.84,3.52,4.02,1.24
4.87,3.93,3.85,3.68,4.25,3.75,3.75
4.58,3.55,3.72,3.97,3.74,3.74,3.74
4.93,4.43,4.21,4.28,4.45,3.70; 3.72

Conditions

Solvent: D2O; Temp.: 30C

Reference

acetone=2.225ppm

Comment

¹³C-NMR

105.0,54.1,70.7,77.4,76.8,61.6
101.9,71.8  81.4  72.2  70.8  17.9
99.9,55.6,70.9,78.9,72.5,61.2
106.3,71.9,73.4,78.7,76.6,61.7
100.2,50.3,78.1,70.0,71.7,61.6

Conditions

Solvent: D2O; Temp.: 30C

Reference

acetone=31.45ppm

Comment

wzx (flippase)

TrEMBL entry

Sequence

MENKKNHIYGIVNNIIVMLSPIIIIPFAIKNVGIDEYGYFVQVNIIYSCLISIFTASLSGYFIKSYLEKK LSFHDIFIIQLFINLFSTAIIGIYILLRFDVVAPYIFVVAMLTNTLNFEWFFHAIGAQKQLLIRNLIIKT MFVIIAIFILEVYKNIAVYFVIFGLNIIIANFLLGIYSFTKIKNKEYILFSNGRGGNRLFCFIKDAKFFF LSPTIGAVYQYGDQILVSLLFPKTALVFINLAKQIIGASVMVSGTLCRVEQKNIFSSEKIERLTKIKKTL MAYVFYLMTTSILIILIGPLILGMLIKETVSLGLNYYILIAGIYVFTSLSIFIDYIIGLTFRTEHYTSIS NICCATIVTLLNAMFLEKYGATFSLFSLFIGELLVFILLSTMHYINIKRRTYYNG

wzy (polymerase)

TrEMBL entry

Sequence

MVNILLCINIIILLICICIYLIALKSKKAPRLFALYLGAFILFIESHIILAITTSFNFGTSEWFFNGEFD YNTKTEVITSINLFIIGMILGSVFIASTITYKSSSYDVTFENKSIARFSWLLLVSILPFVVVYLINLIAF ISSNGFYSLYINGNKISGGYILDLFFLTLYSLLISLKNKKKILFIILCVACVYLFIGTRLEFMFKVFPVL IYYILISKNIHKYFRLKNILAISILFWGLIFSMQYSVSARDNIEMGSNIITTFLKQQGVSVNVIGIAIKD KNNSLLSESVILSPLYDSAISLANSLVGVQSNGNSVEFAENSFSLSHKLSYLEDPSAYLAGYGVGGAAIA ELYIVGGYLACLIGGMLTYIFISILEKIAKKSFFNFIFVMLITGKILYSPRGEFLSFMSADRMLILFLIF TFSYKFLLATSNKKMSFKNE

Comment

From [1]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WclW	1	4	bDGalNAc(1->	->3)aLRha	O117 WbeA (ECODAB) O117 WclW (ECODAB)	ACH97133	[1]
WclX	2	3	aLRha(1->	->4)aDGlcNAc	O117 WclX (ECODAB) O117 WbeB (ECODAB)	ACH97134	[1]
WclY	3	2	aDGlcNAc(1->	->4)bDGal	O117 WclY (ECODAB) O112ac WfeK (ECODAB)	ACH97135	[1]
WclZ	4	1	bDGal(1->	->3)aDGalNAc	O117 WbeD (ECODAB) O117 WclZ (ECODAB) O155 WclG (ECODAB) O104 WbwC (ECODAB) O174 WbcZ (ECODAB) O112ac WfeJ (ECODAB)	ACH97136	[1]

References

[1] Fems Immunol Med Microbiol. 55(1) (2009) 47-54 [PubMed 19040662]

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Thu May 23 06:00:53 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group