Bioorganic Chemistry

Serogroup

O86

Serotype

O86:K62:H2

Strain

ECODAB#

139

Structure

         aDGal(1->3)
                  |
->4)aLFuc(1->2)bDGal(1->3)aDGalNAc(1->3)bDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Identical to

Cross-reacts with

¹H-NMR

5.22 3.89 3.89 3.98 4.20 3.73
4.68 3.89 3.95 4.27 3.65 3.78
5.04 4.23 3.94 4.21 3.86 3.76
4.56 4.14 3.78 4.06 3.66 3.78
5.22 3.78 3.54 3.78 4.33 1.21

Conditions

Solvent: D2O; Temp.: 70C

Reference

acetone=2.212ppm

Comment

1H-NMR [1]

¹³C-NMR

94.3 69.0 70.4 70.3 72.0 62.2
103.2 73.9 75.5 64.7 75.3 61.8
94.1 49.6 77.4 69.8 71.8 61.7
103.6 51.9 75.6 64.5 76.0 61.8
99.7 69.3 70.3 83.4 67.8 16.2

Conditions

Solvent: D2O; Temp.: 70C

Reference

dioxan=67.4ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q4KXD2

Sequence

MIKLLFSSSLLKMGVVLLNLLIPAIIIRFYSEYDFGIYSYLLTYAIVISVLQNGITASFRNLISNLEKND LLDYLLYSVRKITYPFLLIIFCLLLATLYVVGFDTLYGKICFFIAVSLINLYYPVVASYYDATGKTLNFV LLEIIFLLVFIAGVGILIYYKVSIYIVCILTANYRGVYAILLIMKKCVIFKQIRLKKKGGYKIFCSDDMI FIIIQLINVSNTLLFMNMFAKFYGVVAFGVFSVYYRFVAFPQQVISFSSSLIWINFRQVHVNNRVAAMQL LKRLFFIFTLLLIIWGGCVHFFIDKVVYLYSSKPLEYPGVLCFLNIMVCLMLLKDFSSIILNALTLYKEQ MIMNALLCLLNVIFFFFYNETNFDTIYLIFVSLLTLMFVFVNLSLIRSRL

wzy (polymerase)

TrEMBL entry

Q4KXD0

Sequence

MVKIKTPRLLCVIRKEKVGMVISRSNYRNICSYTFFMVNLFILILSVINEGFCEIAYVIISVSSVLFCVI IICLERQGGFLNPMTFCIISVFFFILIRPVFFSQNITENLNEVITAGLEIDEIYVFYSLAVVNISLAFTV LLYSVQKGTVSKLVGQLPDLFFYNKQLSMILLWGGGLFSAIFLIKSYKKFIILGQVSVFEADAYGLHDEL FWFTLSKYCYILSLLFSKNKNFILYSLLIFITSIGYILVGLRGYTIAYGFLLLFFLDIRYRLKIKWLLLV AILVTTISSLILNYRIGIEVNSGLLGIIFNPLLQQGASFETVYGALKYNEKILSCISYYDYFFTNKDIGS CIDIARGVYFKEGGSFASSFYSELIYLGWIIGSVALLLFAFSLAFVQSCYEKIIKNSMNNKLAYTYRLII FLALPNLIYFARSSLFDFITKVLFIALFIGGLSIVRHIALNIKKCH

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbnH	1	1	aDGalNAc(1->	->3)bDGalNAc	O87 WfgO (ECODAB) O22 WbhP (ECODAB) O55:H7 WbgM (ECODAB)	AAV80749	[2]
WbnI	2	3/4	aDGal(1->	->3)bDGal	O86:K2:H2 WcmB (ECODAB)	AAV80756	[2]
WbnJ	3	2	bDGal(1->	->3)aDGalNAc	O86:K2:H2 WcmC (ECODAB) O167 WfdH (ECODAB) O128 WbsK (ECODAB) O113 WbnA (ECODAB) O86:B7 WbwJ (ECODAB)	AAV80758	[2]
WbnK	4	3/4	aLFuc(1->	->2)bDGal	O86:B7 WbwK (ECODAB) O126 WbgL (ECODAB) O55:H7 WbgN(ECODAB) O55:H6 WbgN (ECODAB)	AAV80759	[2]

References

[1] Carbohydr. Res. 185 (1989) 211-223 [PubMed 2471591]

[2] J Am Chem Soc.127(7),(2005) 2040-2041 [PubMed 15713070]

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Sun May 19 17:09:28 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group