Bioorganic Chemistry

Serogroup

O77

Serotype

O77:K96:H-

Strain

ECODAB#

2

Structure

->6)aDMan(1->2)aDMan(1->2)bDMan(1->3)aDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

The DNA sequence of O73, O77 and O106 are almost(>99%) identical. [2]

Cross-reacts with

¹H-NMR

5.32 4.08 4.03 3.77 3.99 3.84 3.84
5.05 4.12 3.86 3.97 3.82 3.55 4.11
4.88 4.08 3.95 3.56 3.76 3.80 3.88
4.76 3.97 3.72 3.65 3.42 3.76 3.94
2.06

Conditions

Solvent: D2O; Temp.: 45C

Reference

TSP=0.00ppm

Comment

1H-NMR [1]

¹³C-NMR

99.9 78.8 70.4 67.0 73.0 61.2
103.0 70.4 71.2 66.4 72.0 65.4
97.6 53.5 80.6 68.8 72.3 61.1
100.4 76.3 74.3 67.3 77.3 61.4
22.6 174.2

Conditions

Solvent: D2O; Temp.: 45C

Reference

dioxan=67.4ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q1L817

Sequence

MIKEIFSDGIFSLFNKICYFGFKTLAIILITRSLGEDVGGEFIFLVGFLEVLRVVTDFGVDLFVIKKYND STDKIKLLNVTFAQKILSGVIVLLLFCLYSFVQGYQYNVYIPIVLALPVALFFNFSNSYFQSLNSNKSLT FPIAIASIATCTIFAIQYFLNVKFNAWDYLCVELLFVSLVTLKLFVSVKFRFIRTSAFDVLMSILSLYKY TLSIGLTAVVVIIYSRIDNFYLKTFAPGDLAAYGQIFRMVDPLVMVSSVFSTVAYARFCRINLQEKNAFT KVFQFLLCIVAYGVLSSLVYFFIIKYLGHFVLISNVNNDFLIMMFLIVACVKCINGGTTAIIQSQGFYKV GLYVSYVCILTSIPTMYFLIMKYGVKGAAMTILLVECISFILLLCSFFMIKKMRPKKTFEM

wzy (polymerase)

TrEMBL entry

Q1L818

Sequence

MHKQYYSLLCNKIQFKHLAIYFSVFLSLSSCFLWGGADTFVSQVYIDILATISTALLVVLMFYYYINRVR YLSLTLLFAFVFSLIIGLPSIYLYYFKNANHGFEIICIWGMLINSILYLQTTETVIKEEKRYLTSLFYIL FSIVALCQLYKILIYLMFILNSGEGHLAIYTESEELLSQVPFFIRAISGFAVVMSLSTFYFKTPTYIKVI AFVLLASDLAIGIRNKFFFSVICIFILYMYSNEYRVRNLFKQLSKPIFLIAGFLCFSMVSYFREGYQIDF MIYIGIVLDTLSSTLGGMQNLFDMQSSGWDQLHPAVVFTQVLPLSGLGFLDDQQIAKDFSIIVLGDVSSG IALSSSGLLESSLLSLNLGVIIYLLYLSGVMLFIKWGLNNQRIIINFVAISMISGFFYAVRGELILPLAF LLKSLPIVLISPLLVKNKIYHGQ

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbaC	1	2, 3	aDMan(1->	->2)aDMan ->2)bDMan	O73 WbaC (ECODAB) O106 WbaC (ECODAB) O7 WbbC (ECODAB)	AAY23735	[2]
WbaD	2	1	bDMan(1->	->3)aDGlcNAc	O73 WbaD (ECODAB) O106 WbaD (ECODAB) O59 WclC (ECODAB)	AAY23736	[2]

References

[1] Carbohydr. Res. 333 (2001) 179-183 [PubMed 11448680]

[2] Microbiology.153 (2007) 2159–2167 [PubMed 17600060]

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Thu Jun 20 02:31:46 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group