Bioorganic Chemistry

Serogroup

O128

Serotype

Strain

ECODAB#

25

Structure

aLFuc(1->2)
         |
  ->6)bDGal(1->3)bDGalNAc(1->4)aDGal(1->3)bDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

4.53 3.99 3.90 4.05 3.68 3.8 3.8
5.08 3.68 3.87 4.11 3.82
4.59 4.03 3.81 4.15 3.66 3.8 3.8
4.61 3.65 3.81 3.90 3.77 3.79 3.79
5.24 3.78 3.66 3.73 4.22 1.21
2.045 2.045

Conditions

Solvent: D2O; Temp.:

Reference

acetone=2.225ppm

Comment

1H-NMR [1]

¹³C-NMR

104.7 52.8 78.2 69.6 75.9 62.4
96.9 69.6 70.5 78.0 71.9 61.5
102.6 51.9 77.2 65.4 76.1 62.4
103.3 77.3 74.9 70.0 74.2 69.3
100.4 69.5 70.9 73.1 68.0 16.5
23.6 23.8 175.25 175.35

Conditions

Solvent: D2O; Temp.:

Reference

acetone=31.45ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q6XQ54

Sequence

MNKKEIIILLIARILQILISLFTMRLMTTILSNEQMGEYYIFLAIYMLASLGIINPIGQY VNRNTYLWWREGVLTQYINRYINFVLCFSLIYVILYGLGECLFKGINTPFWMLFCYLYIV CLSLNQFLLHTLNILNFKIVFAITTLMTSVLSLSGAYLFIEYSIAKQTVSIYYWVAGIII GNFISLCICVVYLRSKNTFQKSILKSINVSIKEVFNFCCPIMITTLFIWFQSSGYRFAVE QMFGLKFLGILAVCFAVSGQIMSVIESLVTQIFQPTLFKHMDNVKNRKQHLDFYINENIA IYASVLFFCTFFMREIFLVLVDNKYLPYFYIGIIALWSEFFRISTNTLSLVFFCEKKMKV GIFPYLIGSLSLAFSIVLFRLLNVNLGILIAFAIGVAQLASFIVCLITVKKISYFKINSI FLMKRIIIVLPSVLIAHCIPKFEMINAIHFIIIFGVGLFYLISLATSFMFIKQPGGVYGS

wzy (polymerase)

TrEMBL entry

Q6XQ50

Sequence

MISYGVLFLFSYINAVLQKRVTVTLLFFIMIIVSFFKYNIGFDWGNYHEIYLQQGDGFFF YEPLFSLLIILSNKVALEYKYFISMCSLITLIVYYSGMRCFARDIKELALALTYFVVSYC YFEHFNIIRQTLAIALFFYSWKYIIETNFIMYSLFIFFASMIHAAAVVLLPFYFFLRIDN FKPVYYGIVVLFIIIVVRFDINSILLSSLNLFKNFDFVARYLANSTDLADFASKKSDLGL SKMYPAIIFVFCCFWGGIRLESRRDYVFFNAGFIAITLLLLSYQVDILFRFFSFFETSFV LLYCIIFSGFNKKSKIILHMVNLIVYTVMTFNMLSVMGLIPYESYIF

Comment

wzx and wzy from O128:B12:H strain ATCC 12810 [2].

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbsH	1	1	aDGal(1->	->3)bDGalNAc	O55:H7 WbgM (ECODAB) O113 WbnE (ECODAB) O3 WclR (ECODAB) O40 WffO (ECODAB) O148 WbbG (ECODAB) O87 WfgO (ECODAB) O22 WbhP (ECODAB)	37788080	[2]
WbsJ	2	4	aLFuc(1->	->2)bDGal	O55:H7 WbgN (ECODAB) O86:K62:H2 WbnK (ECODAB) O86:B7 WbwK (ECODAB) O126 WbgL (ECODAB) O127 WbiQ (ECODAB)	AAO37698	[2]
WbsK	3	2	bDGalNAc(1->	->4)aDGal	O113 WbnA (ECODAB) O55:H7 WbgO (ECODAB) O167 WfdH (ECODAB) O86:K62:H2 WbnJ (ECODAB) O86:K2:H2 WcmC (ECODAB) O86:B7 WbwJ (ECODAB) O127 WbiP (ECODAB) O157:H7 WbdN (ECODAB) O130 WffJ (ECODAB)	AAO37699	[2]
WbsL	4	3	bDGal(1->	->3)bDGalNAc		AAO37700	[2]

References

[1] Carbohydr. Res. 277 (1995) 283-290 [PubMed 8556736]

[2] FEBS Lett. 553 (2003) 99-103 [PubMed 14550554]

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Thu May 23 18:18:34 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group