Bioorganic Chemistry

Serogroup

O22

Serotype

O22:K13

Strain

ECODAB#

26

Structure

->6)aDGlc(1->4)bDGlcA(1->4)bDGalNAc3Ac(1->3)aDGal(1->3)bDGalNAc(1->

CarbBank

LinucsID

9364

Non-sugar components

Acetate

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

5.39 3.43 3.64 3.37 3.76 4.02 3.82
4.43 3.42 3.70 3.75 3.78
4.75 4.17 5.03 4.21 3.71 3.75 3.75
5.06 3.87 3.78 4.15 3.82 3.72 3.72
4.53 4.03 3.80 4.12 3.63 3.70 3.70

Conditions

Solvent: D2O; Temp.: 80C

Reference

acetone=2.225ppm

Comment

1H-NMR [1]

¹³C-NMR

99.3 73.0 74.1 70.5 72.0 69.1
104.4 74.7 77.4 78.0 77.6 174.3
103.9 52.4 74.2 74.3 75.5 61.9
96.6 68.3 80.9 70.2 72.2 62.3
102.9 52.1 76.8 65.4 76.0 62.1

Conditions

Solvent: D2O; Temp.: 80C

Reference

acetone=31.45ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

A7J0Q0

Sequence

MNNNIIDTAGRLKLIAFNFMIFVFLVRVPGFPFIILSIISITILSLFLIKVKVALNNKLIPLYCFVIYMI LRCVIGFVFNGGYEERYFMVLLLMLFVLIVFTVELKDVRLEQCYNGISAAVIVTLLIVLYEIISGNHMST SRYSDINNEAFLWSVSKPTAFYYNENDMLSFVVCFLPFTFVLKNTMLRVAIFLLAAASAIYIGSKAAIIT ILIYGLFTKIISKKAILLLVVTGVVLVNLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDESTSERL SIYKANLDWLGNHPLSATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMITIFGLLFYLLFFSYYFYLWI CLFLKKNKNTACYPLLISSAMYPLIISFGPSSSLKYPFLFILCLLMILGFRNDEKHAKKNI

wzy (polymerase)

TrEMBL entry

A7J0Q3

Sequence

MKINDLLKKYTLFSLSPLLSAFIQLASIFLLGHSLSGPELGIAGIYNTIVFILVFISDGGSSSYFIYRKF LPKYDVNLINIFNVAISAFAIIIIGYYVPEKSDLLGLIVVSLTVTLPLYNYARLLVEKKYNIIAIVELKS KLLFFITLYIAINEFDYQGSAVVMSWAVSYFLRYCLFWWFSKKIELYKNDNLPQNKPAAILKSWWMYIHN QILSQVLNYFTITFDIFIISHFGGLEKVGVYSLCKDATLKISAVLSPVISKLLISHVVNIEENKISPMYK KVYKATIIISISVFGIWALCGSSIIHFIRPQLDQGILTFIIGWSICGILRMMINPIVSIFQATGKTRNEL YVNITSAVSFIFFMGMLSIYFDNIADAVILALICMYSISFVYATVLLRKYFSKIY

Comment

Deposited as DQ851855.1 in genbank. (Unpublished)[2].

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbhP	1	1	aDGal(1->	->3)bDGalNAc	O87 WfgO (ECODAB) O113 WbnE (ECODAB) O128 WbsH (ECODAB) O55:H7 WbgM (ECODAB) O3 WclR (ECODAB) O127 WbiN (ECODAB) O148 WbbG (ECODAB)	ABI34559	[2]
WbhQ	2	2	bDGalNAc(1->	->3)aDGal	O128 WbsK (ECODAB) O167 WfdH (ECODAB) O112ac WfeJ (ECODAB) O113 WbnA (ECODAB) O55:H7 WbgO (ECODAB) O127 WbiP (ECODAB)	ABI34560	[2]
WbhR	3	3	bDGlcA(1->	->4)bDGalNAc	O32 WfeA (ECODAB) O155 WclE (ECODAB) O143 WfdV (ECODAB) O112ab WfeI (ECODAB) O117 WbeA (ECODAB) O107 WclW (ECODAB)	ABI34561	[2]
WbhT	4	4	aDGlc(1->	->4)bDGlcA	O56 WfaQ (ECODAB) O113 WbnE (ECODAB) O87 WfgO (ECODAB) O29 WffY (ECODAB) O152 WfgE (ECODAB)	ABI34564	[2]

References

[1] Carbohydr. Res. 254 (1994) 203-212 [PubMed 7514095]

[2]"Identifying Escherichia coli O22 and O91 serogroups by multiplex PCR of wzx and wzy gene of O-antigen gene cluster." Liu Y., Fratamico P., DebBroy C.Submitted (JUL-2006)

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Sat May 18 15:22:12 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group