Bioorganic Chemistry

Serogroup

O141

Serotype

O141:K85:H4

Strain

ECODAB#

28

Structure

 aLRha(1->3)
          |
   ->4)aDMan(1->3)aDMan6Ac(1->3)bDGlcNAc(1->
          |
bDGlcA(1->2)

CarbBank

LinucsID

Non-sugar components

acetyl group

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

5.20 4.37 4.09 3.95 3.82 3.82 3.82
5.18 4.15 3.90 3.87 3.76 4.28 4.40
4.94 3.98 3.95 3.47 4.25 1.31
4.54 3.79 3.69 3.49 3.44 3.75 3.94
4.52 3.38 3.51 3.62 3.86
2.16 2.04

Conditions

Solvent: D2O; Temp.: 30C

Reference

TSP=0.00ppm

Comment

1H-NMR [1], pD=3.2

¹³C-NMR

101.5 75.6 72.8 72.9 74.2 62.3
102.8 71.4 80.1 67.0 72.6 64.8
98.0 71.8 71.6 73.7 70.3 17.4
102.2 56.2 82.1 72.6 77.6 62.9
104.1 73.8 76.7 73.0 77.3
21.0 23.1 174.1 174.7 174.9

Conditions

Solvent: D2O; Temp.: 30C

Reference

dioxan=67.40ppm

Comment

13C-NMR [1], pD=3.2

wzx (flippase)

TrEMBL entry

A6MEZ8

Sequence

MSKLKSNLVYLTFAQAISYIFPLLTLPYLSHKLDPSDFGMLGVCQAIVQYFIIVTDYGFNITTTKEISIN RNNKKKISKIFSTTLLAKIFLLFVSAIGLIISGWAVKSISGYELLLICCFVGVIGNTFYPLWLFQGTETM KIPVIMSSGAKLLLLLSIFIFVKDRADLNIAALLLNSGNFFAGILGLIYIKTRKLVFWYRPTLQEVYIVL LEGWPVFISTVAISFYTTFNTVLLSYKVSSAEVGFYNAADKIRIAIQSMFGPITQAFYPRIVHLYKVERE AAINLLNKGMLLILGLTIPSALLFFNCSDYIVAHYLSSSFSQTASYLKLLSFLPIIIGIATVYCNWGLLG DGYGKVVSKIYVSFGLIHFLYATPLIIIFKVNGLILSVYITQTLITLTTIYHYYRKVYNGFNK

wzy (polymerase)

TrEMBL entry

A6MF01

Sequence

MEYMLLCFWLAISCCSVKVSLSKINILLIFIFLSGAYSWGYDWIRYREYYENVFLISDLVTWIYEPGFTF ILFVNKLLNLDYHWVVVTCTLILSVNVYKYIKVKNHPNFCLLFVFSIFGFMEFAEQIRQGVAISFILLAV DNLLVKKNKKFIFYVICAAMFHISAIVCLLFLYLVKLLDRKNKAFIMLSLIIWAVFLSLHLMFNIYRFFG VSGFIATKLKGYAESDGSESGILSAGLLLNIMVIVICLLSIQGRHLKYYLSSVFSFFVIQSKIVSIAYRF SYYGYAFIYESFEWLYMANKGRLFNKLAMIIVLLVFAFKPLLNPVYLDMFNDYHFFWFGFLDTLPDVNIT QVKRCSTLLNHGIQYCRR

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfcA	1	3/4	bDGlcA(1->	->2)aDMan	O32 WfeE (ECODAB) O24 WfaO (ECODAB) O22 WbhT (ECODAB)	ABI98974	[2]
WfcB	2	3/4	aLRha(1->	->3)aDMan	O138 WfaF (ECODAB) O135 RfbF (ECODAB) O13 RfbF (ECODAB) O167 WfdK (ECODAB)	ABI98975	[2]
WfcC	3	2	aDMan(1->	->3)aDMan	O59 WclB (ECODAB) O98 WbwV (ECODAB) O8 ORF469 (ECODAB)	ABI98977	[2]
WfcD	4	1	aDMan(1->	->3)bDGlcNAc	O58 WffE (ECODAB) O32 WfeE (ECODAB) O56 WfaQ (ECODAB) O24 WfaO (ECODAB)	ABI98978	[2]

References

[1] Eur. J. Biochem. 254 (1998) 168-171 [PubMed 9652410]

[2] Appl. Environ. Microbiol.(2007) 4082-4088 [PubMed 17449692]

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Sat May 18 16:55:08 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group