Bioorganic Chemistry

Serogroup

O56

Serotype

O56:K-:H-

Strain

ECODAB#

31

Structure

->7)aNeu5Ac(2->3)bDGlc(1->3)bDGlcNAc(1->
                    |
           aDGal(1->2)

CarbBank

LinucsID

9912

Non-sugar components

Comments

Revision [1,2] of previous structure [3]

Identical to

Cross-reacts with

¹H-NMR

2.00 2.51 3.89 4.06 3.73 3.77 3.80 3.50
4.69 3.66 4.16 3.51 3.51 3.98 3.79
4.73 3.65 4.07 3.51 3.57 4.04 3.81
5.52 3.82 3.92 4.00 4.12 3.80 3.80

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

1H-NMR [3]

¹³C-NMR

173.3 101.95 37.45 72.0 53.7 72.6 78.4 69.15 63.2
102.8 56.9 78.2 70.1 76.3 61.9
101.6 73.2 80.2 69.4 75.8 61.9
96.8 69.15 70.1 70.0 71.0 61.9

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

13C-NMR [3]

wzx (flippase)

TrEMBL entry

Q077Q8

Sequence

MKLMKDRFFYFFIELINKGVPFFLLPYVSSAVGVEVYGKIELFTTNYIILSFLFALGFEGWMNSHYFKIS ENNFITILSSFYFLSFLIFFISVITTLLYSSDWTALVIVGYIQSLLNITTLHLRLKGKYIRSGALLLAAS IVNAVMVYVCFELFGKDYNARIEAFLFSSVLLVIFIILCNINIWGGGGKNKINFSFLNKDILLFCLPLCL TMVINWAKGNIDKYFIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKKIILI FLSVLAIFFLLYVIAVCYGFNYVFSADYAPSRNMALLLIFCFLFFSTSSFLINFVLFYGRPKLILLQSLL ITTIHVALSFLLIQKYALFGALFTQVITSILTFISTLLLCYILMKASHK

wzy (polymerase)

TrEMBL entry

Q077Q9

Sequence

MIPYIIFYSLSLTLVVLSLVLKKYDWFWLLFLLFISIIFIGLRVNVGADYTEYVRIYNQSYDTNFELGFD IIFNLFKRFGYDYVFVSLFVFILTSFFFLYSIKSLQHKTLIYFCFLLFMFVPLTSTIRQGLAIPFFIICL LNADRPKVYFMSIAIGCFFHYSILFMLFFFFVRYFKQSYCRAYFIILFFLGLSVFNIVEYMIILLHYIPS LGTLSSKLLMYTQRYNVTLSLTSVAYKLAGLLLVTSFMSFIKINKKLLCSYNLYYISLCLFILFKDNSVF TNRLMYATNISLVTFFSFLLSMMNAFYRKAIVTFLFVIYFSINYFKFTATDLRHSIESAYMPYKSVLIN

Comment

from [4]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfaP	1	1	bDGlc(1->	->3)bDGlcNAc	O152 WfgD (ECODAB) O143 WfdV (ECODAB) O91 WbsA (ECODAB) O112ab WfeI (ECODAB) O21 WclQ (ECODAB)	ABB29913	[4][5]
WfaQ	2	2/3	aDGal(1->	->2)bDGlc	O152 WfgE (ECODAB) O24 WfaO (ECODAB) O29 WffY (ECODAB) O130 WffI (ECODAB) O66 WfaB (ECODAB) O87 WfgO (ECODAB)	ABB29914	[4]
WfaR	3	2/3	aNeu5Ac(2->	->3)bDGlc	O24 WfaN (ECODAB) O104 WbwA (ECODAB)	ABB29915	[4]

References

[1] Carbohydr. Res. 272 (1995) 73-90 [PubMed 7544238]

[2] Eur. J. Biochem. 225 (1994) 1211-1220 [PubMed 7525286]

[3] Carbohydr. Res. 238 (1993) 261-270 [PubMed 7679326]

[4] Current microbiology. 53 (2006) 470-476 [PubMed 17072668]

[5] J. Bacteriol. 190 (2008) 4922-32 [PubMed 18487334]

Edit this entry

Wed May 22 08:38:01 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group