Bioorganic Chemistry

Serogroup

O167

Serotype

O167:H5

Strain

ECODAB#

32

Structure

aDGalf(1->4)
          |
->2)bDGalA6NLAla(1->3)aDGlcNAc(1->2)bDGalf(1->5)bDGalf(1->3)bDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

L-Alanine

Comments

Structure [1]

Identical to

Cross-reacts with

Shigella boydii type 3 [2]

¹H-NMR

5.23 4.16 4.23 4.06 3.86 3.70 3.70
5.03 4.03 4.07 4.15 3.94 3.73 3.73
5.01 4.09 4.00 3.62 3.88 3.85 3.92 2.08 8.47
4.90 4.02 4.23 3.83 3.80 3.66 3.70
4.68 3.82 3.66 3.48 3.44 3.76 3.91 2.05 8.21
4.65 3.59 3.75 4.25 4.21 1.35 7.65 4.16

Conditions

Solvent: D2O; Temp.: 60C

Reference

TSP=0.00ppm

Comment

1H-NMR [1], pD=5.8. Amid protons measured in H2O/D20 9:1 10C

¹³C-NMR

106.7 87.6 75.8 82.8 70.9 63.7
109.0 82.0 77.3 82.5 76.8 62.3
97.6 53.3 79.7 69.5 73.2 61.5 23.1 174.4
103.7 76.6 73.5 81.2 70.2 63.8
102.5 55.8 81.7 69.2 76.0 61.9 23.1 175.0
101.7 79.5 73.3 79.4 74.2 169.1 18.0 180.0 51.3

Conditions

Solvent: D2O; Temp.: 60C

Reference

acetone=31.00ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

B5L3V7

Sequence

MVKTDLTVFKNIISLFSIRFAGYIIPLITLPYLVKVLQPVGFGYLSFGIAVLQYCTLVVNYGFDLSATRK IAQNRENVHYISDIFWNILFLRLFISLVGFLVLVVFIHLFPDLYRIKWILLWGYLSVLGTALFPMWLFQG KEQLGRISAARIFLQISCVPLFFVFVKNTQDDWVASLIYALPQLLISVVAVYIIHKRKWIVLKKIKLATI KKELIDGWHLFISTAAISLYTTTITVVLGIVPGPYSVAIYTSANKILQAAQGLYQPISSAFYPRINNLIK ENKQLAFKTIRKLFKIQFVITICISVGLFLFSNIVVDILYGDDYRNAALILQLLSPIPIVVGCSNIFGIQ ILLTCGYKKEFSTILLISGLINMILIFPLCYKFNEFGAAFSVLITEIFVTVSMLFVVILKKIPVWESKRN V

wzy (polymerase)

TrEMBL entry

B5L3W2

Sequence

MNNTLRQYSLVQVFITMYCLFPFYFHNIYIIQTFSLYSLALSFVVYTFFYKVRFVSRSGAITILASTTTF LIFLFMSMLINLSSDMTFVYEQVFGFSKQLLLALVPFCLYFSFDSDDINYAVHSILLLFIRAISLYVFIS VIFLIPEVRNYWIDIIYFSSDQNKELINSASYYTRFGLQGFSGFRHAILCAIALIFSIYLFMCGYKKIKN YWMSVFLVCIGCFLYGRIAIAVILCITLISLFYLLCKFEKLYFIFIVCLCVGTIACVVYMNIDYIQDSYS LAWIFEPLINFINEGQFTSHSTNDLSSMYIMPNENTMLLGDGKYTNHDGSYYMHTDVGFLRPVFFGGIFF VFFYYMMLILPLILIIKNSGLERRNILTLFLALALIVFELKGESFISFSAIIFSFILIPIICKRLN

Comment

From [3]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfdF	1	3	aDGlcNAc(1->	->2)bDGalf	O56 WfaQ (ECODAB) O29 WffZ (ECODAB) O138 WfaH (ECODAB) O87 WfgN (ECODAB)	187880527	[3]
WfdG	2	4	bDGalA(1->	->3)aDGlcNAc	O143 WfdR (ECODAB)	187880528	[3]
WfdH	3	5	aDGalf(1->	->4)bDGalA	O143 WfdT (ECODAB) O128 WbsK (ECODAB) O22 WbhQ (ECODAB) O86 WcmC (ECODAB) O86:K62:H2 WbnJ (ECODAB) O86:B7 WbwJ (ECODAB) O127 WbiP (ECODAB) O113 WbnA (ECODAB)	187880529	[3]
WfdI	4	2	bDGalf(1->	->5)bDGalf	O104 WbwB (ECODAB) O56 WfaQ (ECODAB) O87 WfgO (ECODAB)	187880531	[3]
WfdJ	5	1	bDGalf(1->	->3)bDGlcNAc	O167 WfdK (ECODAB) O42 WbeY (ECODAB) O28ac WbeY (ECODAB) O146 WbwV (ECODAB)	ACD37048	[3] Same linkage as WfdK?
WfdK	6	1	bDGalf(1->	->3)bDGlcNAc	O42 WbeY (ECODAB) O28ac WbeY (ECODAB) O167 WfdJ (ECODAB) O146 WbwV (ECODAB)	187880533	[3] Same linkage as WfdJ?

References

[1] Eur. J. Biochem. 246 (1997) 565-573 [PubMed 9208951]

[2] J. Clin. Microbiol. 17 (1983) 521-523 [PubMed 6341404]

[3] FEMS Microbiol.(2008) 627-653 [PubMed 18422615]

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Wed May 22 13:11:24 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group