Bioorganic Chemistry

Serogroup

O21

Serotype

O21:K20:H-

Strain

ECODAB#

34

Structure

bDGal(1->4)
         |
  ->3)bDGal(1->4)bDGlc(1->3)bDGalNAc(1->
                    |
        bDGlcNAc(1->2)

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

E. coli strain 105 [1], Hafnia alvei O39 [1]

Cross-reacts with

¹H-NMR

4.87 3.72 3.54 3.46 3.38 3.77 3.92
4.86 3.51 3.71 3.93 3.68 3.78 3.78
4.78 3.97 3.94 4.20 3.69 3.79 3.79
4.61 3.60 3.68 3.67 3.54 3.81 3.93
4.47 3.74 3.83 4.38 3.74 3.77 3.82
2.08 2.06

Conditions

Solvent: D2O; Temp.: 75C

Reference

TSP=0.00ppm

Comment

1H-NMR [1], pD=6.9

¹³C-NMR

102.2 56.8 75.1 70.9 76.8 61.8
103.6 72.4 73.9 69.7 75.8 61.9
104.0 52.5 80.6 68.7 75.5 62.0
103.0 79.9 76.5 79.5 75.3 60.9
103.9 71.3 82.5 76.1 75.3 61.6
23.8 23.1 175.4 175.2

Conditions

Solvent: D2O; Temp.: 75C

Reference

dioxan=67.4ppm

Comment

13C-NMR [1], pD=6.9

wzx (flippase)

TrEMBL entry

B5T081

Sequence

MFTIKTVFVLRIFGAILALSTSVMISRTLNMSEAGGVFFLLSVTGIISSLSSLGQNNLILKKCASVKYAL KARGVFFLRCIKRSVISSIILSIILIPVIHYFSPQLLLRNTWCLAILLIISSSVNILLYSFLQSQGLVTF SVVLQYIFQPLLFIFFVLLIVLIKNESVLLVSLSYFFSIILIGGGGGVYSYYKFRDDFNQLETESIPFRL KELSEYFIGNSLGMLIVQSYVVLSGLLLPAADVAIIAVSDRISLVINLFAMSISTILAPKVASLYSQSKM NEIKELTKKAMFFIMIPCVLMALLFPFFSGLILSIFGVQYSNASEVLIILVMTQIINAIFCPVYVFLNMS DRQGFISKLHIYMLIPSLVITFYLTNIFGVVGIAVSKLIVVSLINIIPLIYCILLSNRVIK

wzy (polymerase)

TrEMBL entry

B5T085

Sequence

MIYIIINTIVLFYMFLFNAYNRIPYGKLIIGGICVFFMTLLPAFQYGVGTDYFSYQNIYNNANELDTFYQ NKEYLFYGYMRLYQLTGWGFKGFIALTALLQSLLVFVIVFQLWKNYGYSLVLVFFLFWVVTNLFHTQMNI IRASFSIYLFAISILYKFRGKLLLSFILMVAALGFHRSALIGFCFLVIPDKMYFFACKHAFKIYVLTFLL FLFSYLQQIIYYIVQNLFPYYSHYLSSFDESSVSIMNVLTKMYWIPFNLLFLILLRLKVFVIKDNERKLI GMWALTVNVYLLMLSFDFISRVNYYFVIFYIIPIIYVIKYTVKNRYFVCLYLSLLYCFIPYLLKVILFPI AEFYYKSYLF

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WclN	1	4	bDGal(1->	->4)bDGal	O32 WfeB (ECODAB) O121 WbqE (ECODAB) O130 WffH (ECODAB)	203285043	[2]
WclO	2	2/3	bDGlcNAc(1->	->2)bDGlc	O112ab WfeF (ECODAB) O124 WfeS (ECODAB)	ACH97160	[2]
WclP	3	2/3	bDGal(1->	->4)bDGlc	Beta1,4-galactosyltransferase WaaX, E. coli O172 Orf3 (ECODAB) O40 WffP (ECODAB)	ACH97161	[2]
WclQ	4	1	bDGlc(1->	->3)bDGalNAc	O130 WffJ (ECODAB) O157:H7 WbdN (ECODAB) O112ab WfeI (ECODAB) O143 WfdV (ECODAB) O152 WfgD (ECODAB) O91 WbsA (ECODAB)	ACH97163	[2]

References

[1] Eur. J. Biochem. 266 (1999) 241-245 [PubMed 10542071]

[2] J Microbiol Methods.75(2) (2008) 329-34 [PubMed 18700154]

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Thu May 23 13:12:47 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group