Bioorganic Chemistry

Serogroup

O127

Serotype

O127a:H-

Strain

ECODAB#

40

Structure

->2)aLFuc(1->2)bDGal(1->3)aDGalNAc(1->3)aDGalNAc(1->

CarbBank

LinucsID

4837

Non-sugar components

Comments

Strcuture [1]

Identical to

Cross-reacts with

Blood-group H [2]

¹H-NMR

5.34 3.95 3.85 3.69 4.29 1.21
5.24 4.40 3.96 4.14 4.14 3.77 3.77
5.10 4.27 3.97 4.24 3.77 3.77 3.77
4.62 3.75 3.79 3.93 3.67 3.77 3.77
2.05 2.05

Conditions

Solvent: D2O; Temp.: 70C

Reference

TSP=0.00ppm

Comment

1H-NMR [1]

¹³C-NMR

99.17 71.59 70.73 73.27 67.68 16.12
99.47 48.92 74.11 65.54 74.19 62.06
94.03 49.70 75.66 69.73 71.92 61.78
103.15 76.49 74.66 69.95 75.66 61.67
175.5 175.5 23.0 23.0

Conditions

Solvent: D2O; Temp.: 70C

Reference

acetone=31.07ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q5J7D0

Sequence

MSIISSFKNNIFLRNTMFNYIGMIWMGGGIIVLIPFYIKLLGPHGWGIVSACLSIQAMLL ILDAGCSQIMPRDIAKTNTPENIFFAYFWIYIAVAFVGAVFLTSSAGWLAFRWFNAGHSS RELEFCIRLLAIQFLFQFANNLNIGFWNGTQKQFNTNLSQVCFFSCKHCVALISLMVNPE PIVYIVSFAVVTCIEFIFNFLWITRNNIDLKGLIDNNILIQLIDLVKENYIFSFGVIVGV FVSQLDKILLSHYVETQLYGVYVIVAQLGLSFLQLQYPLMKSVLPALSSQGEKSIFNVLS LKKMIIMLLVLAFPIGLTIIFSSDILYLWTRNHYIANHGGGALALICLSVLINIPYTFIY TNLVRLNAGGIILFSNIISALSMFILFHFMKENNSISIGGMMWIAYASISFSIGGIFLLM RAR

wzy (polymerase)

TrEMBL entry

Q5J7C8

Sequence

MMQITIPKSNDFRRFFNPVILILMSIVLNTFFQLYTSEEEWNSFFIFNQKPDYTVFFISL VFYLFFLFGAFFAYLFNYQKHSNHRSHYLYNFSFYNGLLKFVFYSNVISLIVTQYYMHEA NIYNIYISGGVSAQTIESQIASSPLGIHGISVMLGYFGIIMYGISRLIKDNRAIVFISIL VIIIKFISYAKLQSLLYVLLALMLFSRRRISINKGVIIGGVVIFLFSFTRIIRNPEQNLS LSFDFLFRFVGGFYFGSPVVNFNYVIQNNIQDVFYFFNWFLPQKIIPASPISLEFPDVTS PIGFVGSAYVSLGIFSVIYAYLVGFIIQLIYLQRNTSPFAFVFQPFVIMACIFAMMYNNF VNMIFFILPLIFSYLVVIKTICARKEDV

Comment

Deposited as AY493508 [3] (unpublished).

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbiN	1	1	aDGalNAc(1->	->3)aDGalNAc	O87 WfgO (ECODAB) O22 WbhP (ECODAB) O128 WbsH (ECODAB) O55 WbgM (ECODAB)	AAR90884	[3]
WbiP	2	2	bDGal(1->	->3)aDGalNAc	O86 WbnJ (ECODAB) O86 WcmC (ECODAB) O55:H7 WbgO (ECODAB) O167 WfdH (ECODAB) O128 WbsK (ECODAB) O147 WfbZ (ECODAB)	AAR90893	[3]
WbiQ	3	3	aLFuc(1->	->2)bDGal	O86 WbnK (ECODAB) O86 WbwK (ECODAB) O55 WbgN (ECODAB) O128 WbsJ (ECODAB) O126 WbgL (ECODAB)	AAR90894	[3]

References

[1] Carbohydr. Res. 247 (1993) 255-262 [PubMed 7693348]

[2] J. Exptl. Med. 113 (1961) 1077-1093

[3] Yi W., Shao J., Wang P.G.; "Sequence and characterization of Escherichia coli O127 antigen biosynthesis gene cluster" (Unpublished, 2003).

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Mon May 20 08:00:27 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group