Bioorganic Chemistry

Serogroup

O143

Serotype

O143:K-:H-

Strain

ECODAB#

43

Structure

->2)bDGalA6NHCH(CH2OH)2,3/4Ac(1->3)aDGalNAc(1->4)bDGlcA(1->3)bDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

acetate, 2-amino-propan-1,3-diol

Comments

Structure [1]. 0.3 acetates/repeating unit at position 3, 0.5 acetates/repeating unit at position 4

Identical to

Cross-reacts with

Shigella boydii type 8 [2]

¹H-NMR

5.49 4.35 4.04 4.26 4.00 3.73 3.76 2.11
4.90 3.85 3.75 3.51 3.40 3.73 3.92 2.07
4.66 3.77 3.79 4.19 4.25 3.7 3.7 4.05 3.7 3.7
4.50 3.38 3.71 3.81 3.81

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

1H-NMR [1]

¹³C-NMR

97.6 48.9 78.6 69.4 71.0 61.6 23.4 174.6
101.8 55.5 83.5 69.6 76.2 61.8 23.1 175.4
103.4 77.4 74.6 70.1 75.3 170.9 61.3 53.2 61.4
103.6 74.0 77.2 76.1 77.4 175.6

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

B5L391

Sequence

MSSFLRSIISVSALKLVDYIVPIIIMPLVLTRFNLEGYGVYSYVLTISTFISFIYDACLNTVGVQQLNKL KGNEIAQQSFVISAIFFKLVFFFVCAMVFIIISETAGKSDYFCVVIFSFGFGMLNSWYYIAKDNFRFIII VSLVVKILAVLFVLYYAFELHMFIFIQAIASALVGLISLIKIKNDNRLGKISFRHIKNYLKNIKHMYIYS GVNALIQPIINSAILASGNASLLGIYNVVMRFVTVSVTFSNSIITVLNKCNSEVFYSVGHKTNYVEVKYR SQLLKLLFYSIVVFICVAAISYVSIYLTKGEISRFFIFLIFSFSLAVIPSILNQYLTQLIHMIHSSKNIV KYVICSNVLSLISIFLFSSLFPYYYIVYAVVLSPIIITICCLWDLKCKNLL

wzy (polymerase)

TrEMBL entry

B5L394

Sequence

MKVFFDVFLLFLFVFAFSIPVFFNSGYLIYIFSLIYLITNIRIRTVFFSLISTRIFFIAFLSLACMLFFS CISLVLNGTGDLSIMGVIFNNFAALFSSVIIASHLIQHNNDNVLKLFFLIALLQSFFIILMMFFPSINAY IQSIIRTKEEIEFMSNTYGGIRGLGISGSVAFGLAIIMSLLSFLSIVWFNEEDCNIPFFFKLFFIILLLF ASISAGRTAVLGFVLGGVYSFICSDYKKKFYRALQILIYVSLFLFIFWRFFLNEHVYDILKKYSDYSFQM LYYYLDSGKLSTTSTDALFNMYFPLTEKQIFIGDGYYTVKGWDILSRDRCWIYAVSIIFGCGTIGYFLFF IFYFFDYLCC

Comment

From [3]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfdR	1	3	bDGalA(1->	->3)aDGalNAc	O167 WfdG (ECODAB)	ACA24759	[3]
WfdT	2	2	aDGalNAc(1->	->4)bDGlcA	O167 WfdH (ECODAB) O128 WbsK (ECODAB) O55:H7 WbgO (ECODAB) O127 WbiP (ECODAB) O114 WbuP (ECODAB) O130 WffH (ECODAB)	ACA24762
WfdU	3				O168 WfeW (ECODAB) O141 WfcC (ECODAB)	ACA24763	[3]
WfdV	4	1	bDGlcA(1->	->3)bDGlcNAc	O112ab WfeI (ECODAB) O91 WbsA (ECODAB) O157 WbdN (ECODAB) O79 WbdX (ECODAB) O130 WffJ (ECODAB) O152 WfgD (ECODAB) O21 WclQ (ECODAB) O56 WfaP (ECODAB)	ACA24766	[3]

References

[1] Carbohydr. Res. 291 (1996) 209-216 [PubMed 8864232]

[2] Methods Microbiol. 14 (1984) 43-112

[3] FEMS Microbiol.(2008) 627-653 [PubMed 18422615]

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Thu Jun 20 09:54:02 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group