Bioorganic Chemistry

Serogroup

O91

Serotype

O91:K-:H-

Strain

ECODAB#

45

Structure

->4)aDQui3NAcyl(1->4)bDGal(1->4)bDGlcNAc(1->4)bDGlcA6NGly(1->3)bDGlcNAc(1->

Acyl=(R)-3-hydroxybutyryl

CarbBank

LinucsID

Non-sugar components

(R)-3-hydroxybutyric acid Glycine

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

4.52 3.67 3.75 4.04 3.78 3.88 3.81
4.54 3.42 3.66 3.90 3.97 3.84
4.58 3.74 3.81 3.47 3.46 3.77 3.98 2.03
4.58 3.74 3.72 3.72 3.63 3.82 4.02 2.03
4.87 3.67 4.08 3.44 4.27 1.23 2.46 2.53 4.24 1.26

Conditions

Solvent: D2O; Temp.: 65C

Reference

TSP=0.00ppm

Comment

1H-NMR [1], pD=9.0

¹³C-NMR

104.1 71.7 73.1 77.4 76.3 61.1
103.7 73.3 74.6 79.4 75.3 169.9 176.8 44.5
101.6 56.1 83.0 69.8 76.4 62.2 175.3 23.1
101.1 56.1 73.2 79.9 75.9 75.7 61.1 175.3 23.2
99.8 71.3 53.3 80.5 68.8 17.1 46.3 65.8 22.6

Conditions

Solvent: D2O; Temp.: 65C

Reference

acetone=31.00ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q8GMJ7

Sequence

MRMLKTGLLNFISVLVKVITGLFLNKIMALYVGPAGYALVSQFQNGINFITTFASGAINT GVTKYTAEYANDKGKTSDLFKCITLLAITCCSFFSLTIIVFSEYFSLKLLGTINYSGVIK WFAATLFLFVANSILVSIINGLKEFKLYVLINIFGSLFSLLFSGILIYFFNLKGALIAFA TNQSIVFFMTFYLIISKKMVSLKSFIGMPSDFYFIKKLIQYALMAIVSAFCVIYMQIIIR TYIANHFGWVQAGIWDGVWRISSLYLMLVTVPLGVYYLPRLAELHTKSLLLREIKQGYLF LLPIIIISAIIIYYLRYFIIELLFSKSFVSMGDIIGWQLLGDIFKIGSWLLSYIMLSKAL TKTYIITEITTALLFLGGVHYLSPLYGLKGVTLAYCITYFCYWIMMIVVIISYFKGNFRN E

wzy (polymerase)

TrEMBL entry

Q8GMJ3

Sequence

MKASSVLTPYLNWFYGFLCFLSLWPFFSWKWPAIPVSFIAICFFLFLFDLCYINKLKVNK KIFLIIFSTLFVLILFILLPGGIPPWFNYYSLFLFLFLLLPRKRIFEISLKFRSIFIFSL LPGLVIYALLIIGVKLPYGILDAHNELKDSLGIYYRDYIGTVALSHLVLTVGDSTIIRFS GMLDEPGLLGTISALLLLADKLNFKHKSNYVLLLSGVISISLAFYLLILMGLIFQTRRKI ISLAIVFLIGIAIYHSPFYENFLQKRISDSESGLVFRDNRISDCFKEYYTAFENDSELNL FFGIGNNAHLRTGCDVSSYKMYIYDYGYLGFITIALLQIMQYMIPIMGGYACKFLKASFV FICLFILSYYQRPIFFNLAFSMIFYYSIFTYLKTVGISATNKK

Comment

From strain ECA95 [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbsA	1	1	bDGlcA6NGly(1->	->3)bDGlcNAc	O79 WbdX (ECODAB) O143 WfdV (ECODAB) O112ab WfeI (ECODAB) O24 WbdN (ECODAB) O152 WfgD (ECODAB) O157:H7 WbdN (ECODAB)	AAK60450	[2]
WbsE	2	4	aDQui3NAcyl(1->	->4)bDGal		AAK60455	[2]
WbsF	3	2	bDGlcNAc(1->	->4)bDGlcA6NGly	O112ab WfeF (ECODAB) O112ac WfeM (ECODAB)	AAK60456	[2]
WbsG	4	3	bDGal(1->	->4)bDGlcNAc	O32 WfeA (ECODAB)	AAK60457	[2]

References

[1] Biochemistry 38 (1999) 12205-12211 [PubMed 10493787]

[2] J. Appl. Microbiol. 93 (2002) 758-764 [PubMed 12392520]

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Tue May 21 09:33:15 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group