Bioorganic Chemistry

Serogroup

O152

Serotype

O152:K?:H?

Strain

ECODAB#

5

Structure

bLRha(1->4)
         |
  ->3)aDGlcNAc(1->6)aDGlc(1->2)bDGlc(1->3)bDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

Phosphorous

Comments

Structure [1]

Identical to

Shigella dysenteriae 12 [2]

Cross-reacts with

¹H-NMR

5.37 4.07 4.22 3.81 3.95 3.84 3.94
5.27 3.57 3.75 3.51 4.12 4.09
4.91 4.23 3.64 3.38 3.44 1.33
4.75 3.64 4.11 3.58 3.47 3.78 3.98
4.71 3.45 3.55 3.43 3.43 3.71 3.92

Conditions

Solvent: D2O; Temp.: 50C

Reference

TSP=0.00ppm

Comment

1H-NMR [1]

¹³C-NMR

95.0 54.2 75.5 75.7 72.4 61.2
98.9 72.4 73.5 69.9 71.6 65.2
102.2 71.1 73.5 73.0 73.0 17.7
101.0 56.9 79.5 69.3 76.2 61.9
101.6 78.7 75.7 70.7 76.8 61.6

Conditions

Solvent: D2O; Temp.: 50C

Reference

dioxan=67.4ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

B5L3E9

Sequence

MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRD NHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSA ILEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFC YKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLS NIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQISSFNILNVLLIGFFFNALAMIP FSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN

wzy (polymerase)

TrEMBL entry

B5L3F1

Sequence

MLFTYASYLCILVFSTSVAFCYEHSKSNFKVLLWSVLFLSLWIPAAIRFGVGADYFRYLSMVESVRLGYI STEVGFYFINYFVVWFDLNPQWSIAISSFIVIFLSLKAIPRNYFSISVFVLVCTFYLPSFSLVRQAIAIA FIAYAVKNYIDGNKKNYIIFILIGSLFHLSALLLVPIYFIARFRVRSSILFLGILIISLLLFLGSGLNAL LNSEFFLQSKYGYYATSDFIQDAKIGSGVGVFIKMLLPLIFITNAKKILKTNQNLNILLWMSVGCVLANV CSMKIHIFNRLADIFLFVNFMVTSYIINSLEKNVIKQLSSMAIIIVFIVFYCRTIQANYNDDLGGIGISP YKTILSK

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfgB	1	4	bLRha(1->	->4)aDGlcNAc	O105 WbsX (ECODAB)	ACA24818	[2]
WfgC	2	3	aDGlcNAc(1->	->6)aDGlc		ACA24820	[2]
WfgD	3	1	bDGlc(1->	->3)bDGlcNAc	O56 WfaP (ECODAB) O143 WfdV (ECODAB) O112ab WfeI (ECODAB) O130 WffJ (ECODAB) O21 WclQ (ECODAB) O79 WbdX (ECODAB)	ACA24822	[2][3]
WfgE	4	2	aDGlc(1->	->2)bDGlc	O24 WfaO (ECODAB) O56 WfaQ (ECODAB) O29 WffY (ECODAB) O66 WfaB (ECODAB) O130 WffI (ECODAB)	ACA24823	[2]

References

[1] Carbohydr. Res. 340 (2005) 167-171 [PubMed 15620681]

[2] FEMS Microbiol Rev.(2008) 627-653 [PubMed 18422615]

[3] J. Bacteriol. 190 (2008) 4922-32 [PubMed 18487334]

Edit this entry

Sat May 18 22:56:45 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group