Bioorganic Chemistry

Serogroup

O2

Serotype

Strain

ECODAB#

51

Structure

      ->3)aLRha(1->2)aLRha(1->3)bLRha(1->4)bDGlcNAc(1->
             |
aDFuc3NAc(1->2)

CarbBank

LinucsID

4845

Non-sugar components

Comments

Structure [1]

Identical to

Cross-reacts with

O74

¹H-NMR

5.16 4.08 3.95 3.46 3.82 1.29
5.05 4.24 3.97 3.66 3.71 1.26
4.96 3.79 4.17 3.77 4.52 1.22
4.87 4.13 3.61 3.50 3.41 1.24
4.79 3.73 3.67 3.63 3.52 3.96 3.84

Conditions

Solvent: D2O; Temp.: 70C

Reference

TSP=0.00ppm

Comment

1H-NMR [1]

¹³C-NMR

101.6 78.7 71.0 73.3 70.0 17.6
100.3 76.3 78.2 72.6 70.6 17.5
97.8 67.1 52.0 71.5 67.9 16.2 23.1
101.3 71.4 80.8 72.4 73.0 17.3
103.2 56.9 75.0 78.4 75.4 62.1 23.0

Conditions

Solvent: D2O; Temp.: 70C

Reference

dioxan=67.4ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

B3U3P9

Sequence

MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQVQSLITIVAGTTSAPVSTGLVRYTAEN WQEGQEACAPWWRACLRVTLFLFLLIIPVVIILSKNISELLFSDGQYTWLIIFACCILPFSIINTLIASV LNGQQFYKQYILVGMFSVFISTMFMILLIVAYNLKGALIATAINSAIAGLVLVLFCLNKSWFRFKYWWGK TDKDKIIKIIHYTLMALVSVISMPTALMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALSTYFLP RLTIIKTSFLIKKEVNSTILYIISITSFMALSIYLFRDLVITVLFTEQFRSARELFLLQLIGDVIKIAGF LYAYPLQSQGHTKLFISSEVIFSMLFIITTYIFVVNYGVHGANISYVITYSLYFVFAFVFTNFINVRRNN

wzy (polymerase)

TrEMBL entry

B3U3Q2

Sequence

MTENSPKYKHDKSLISFIYLFFIFTLIVGFIIANTQFLGRSRDYDNYIQIFSGKEGEGVLELFYRGLMLI TTSYETIIFIILTCSFFIKARFLANYSRNFSGLTLFFIYYASVALWVLDYTQFRNGLCISILMFSVYYLF INKPTYFYFSVLCAIATHWSALPFLLLYPFVYSTKIRRLGYFCFSILVLIAISGEGKEIISFIRNFGVGQ KIGNEAGVNLINSLSLTAISWFIISYISSIGNERRNLRLFFCYGVMQYVTFSLFSLPVMAFRILEMYFFL MLTIGVFIKQKKNYYFVFCKVLILLYLTYYYHMVFGVINV

Comment

Deposited as EU549863[2] (unpublished).

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
unnamed	1	4	aDFuc3NAc(1->	->2)aLRha	O141 WfcA (ECODAB)	ACD75807	[2]
WbbQ	2	2, 3	aLRha(1->	->3)bLRha ->2)aLRha	O135 RfbF (ECODAB) O13 RfbF (ECODAB) O150 WfgI (ECODAB) O148 WbbQ (ECODAB) O147 WfbY (ECODAB) O139 WfaI (ECODAB) O146 WbwV (ECODAB) O177 WbdY (ECODAB)	ACD75810	[2]
WbuV	3	1	bLRha(1->	->4)bDGlcNAc	O149 WbuV (ECODAB)	ACD75811	[2]

References

[1]Carbohydr. Res. 161 (1987) 273-279 [PubMed 3555792]

[2]Yan,X. and Fratamico,P."Escherichia coli serogroup O2 O-antigen gene cluster.";Submitted (MAR-2008)

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Sat May 25 17:33:28 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group