Bioorganic Chemistry

Serogroup

O117

Serotype

O117:K98:H4

Strain

ECODAB#

6

Structure

->4)bDGalNAc(1->3)aLRha(1->4)aDGlc(1->4)bDGal(1->3)aDGalNAc(1->

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

4.97 4.45 4.19 4.30 4.42 3.73 3.73 2.08
4.96 3.58 3.84 3.59 4.18 3.81 3.73
4.91 4.17 3.86 3.56 4.03 1.27
4.72 4.03 3.84 4.06 3.76 3.90 3.85 2.06
4.60 3.57 3.73 4.04 3.76 3.88 3.82

Conditions

Solvent: D2O; Temp.: 65C

Reference

acetone=2.230ppm

Comment

1H-NMR [1]

¹³C-NMR

99.54 49.72 77.89 69.35 71.37 61.56 23.19 175.19
100.88 72.88 72.41 78.43 71.97 61.08
101.29 71.27 81.03 71.89 70.30 17.43
104.16 53.70 71.37 76.93 75.91 61.17 22.99 176.01
105.60 71.76 73.16 78.61 76.09 61.01

Conditions

Solvent: D2O; Temp.: 65C

Reference

acetone=31.07ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

A6M9B3

Sequence

MENKKNHIYGIVNNIIVMLSPIIIIPFAIKNVGIDEYGYFVQVNIIYSCLISIFTASLSGYFIKSYLEKK LSFHDIFIIQLFINLFSTAIIGIYILLRFDVVAPYIFVVAMLTNTLNFEWFFHAIGAQKQLLIRNLIIKT MFVIIAIFILEVYKNIAVYFVIFGLNIIIANFLLGIYSFTKIKNKEYILFSNGWGGNRLFCFIKDAKFFF LSPTIGAVYQYGDQILVSLLFPKTALVFINLAKQIIGASVMVSGTLCRVEQKNIFSSEKIERLTKIKKTL MAYVFYLMTTSILIILIGPLILGMLIKETVSLGLNYYILIAGIYVFTSLSIFIDYIIGLTFRTEHYTSIS NICCATIVTLLNAMFLEKYGATFSLFSLFIGELLVFILLSTMHYINIKRRTYYNG

wzy (polymerase)

TrEMBL entry

A6M9B4

Sequence

MVNILLCINMIILLICICIYLIALKSKKAPRLFALYLGAFILFIESHIILAITTSFNFGTSEWFFNGEFD YNTKTEVITSINLFIIGMILGSVFIASTITYKSSSYDVTFENKSIARFSWLLLVSILPFVVVYLIKLIAF ISSNGFYSLYINGNKISGGYILDLFFLTLYSLLISLKNKKKILFIILCVACVYLFIGTRLEFMFKVFPVL IYYILISKNIHKYFRLKNILAISILFWGLIFSMQYSVSARDNIEMGSNIITTFLKQQGVSVNVIGIAIKD KNNSLLSESVILSPLYDSAISLANSLVGVQSNGNSVEFAENSFSLSHKLSYLEDPSAYLAGYGVGGAAIA ELYIVGGYLACLIGGMLTYIFISILEKIAKKSFFNFIFVMLITGKILYSPRGEFLSFMSADRMLILFLIF TFSYKFLLATSNKKMSFKNE

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbeA	1	4	bDGalNAc(1->	->3)aLRha	O107 WclW (ECODAB)	ABE98414	[2]
WbeB	2	3	aLRha(1->	->4)aDGlc	O107 WclX (ECODAB) O4 WbuG (ECODAB)	ABE98415	[2]
WbeC	3	2	aDGlc(1->	->4)bDGal	O107 WclY (ECODAB) O112ac WfeK (ECODAB)	ABE98416	[2]
WbeD	4	1	bDGal(1->	->3)aDGalNAc	O107 WclZ (ECODAB) O155 WclG (ECODAB) O104 WbwC (ECODAB) O174 WbcZ (ECODAB)	ABE98417	[2]

References

[1] Carbohydr. Res. 323 (2000) 103-110 [PubMed 10782292]

[2] Mol Cell Probes.(2007)295-302 [PubMed 17452091]

Edit this entry

Sun May 19 04:44:41 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group