Bioorganic Chemistry

Serogroup

O24

Serotype

O24:K:H-

Strain

ECODAB#

61

Structure

->7)aNeu5Ac(2->3)bDGlc(1->3)bDGalNAc(1->
                    |
           aDGlc(1->2)

CarbBank

LinucsID

4849

Non-sugar components

Comments

Revision [1,2] of previous structure [3]

Identical to

Very similar to O56

Cross-reacts with

¹H-NMR

4.74 3.63 4.15 3.52 3.54 3.77 3.65
4.61 3.94 4.04 4.15 3.75 3.84 3.84
1.99 2.59 4.15 3.777 4.04 3.74 3.81 3.80 3.56
5.44 3.49 3.85 3.30 3.88 3.85 3.70

Conditions

Solvent: D2O; Temp.:

Reference

acetone=2.225ppm

Comment

1H-NMR [1], pD=2

¹³C-NMR

104.7 73.1 78.75 70.5 76.5 62.2
103.1 53.2 79.8 69.4 75.6 62.9
173.9 102.35 37.9 68.9 54.7 72.3 78.9 73.1 63.6
96.7 72.95 74.3 71.4 72.65 62.0

Conditions

Solvent: D2O; Temp.:

Reference

acetone=31.45ppm

Comment

13C-NMR [1], pD=2

wzx (flippase)

TrEMBL entry

Q077R8

Sequence

MKLLKSRFSYFATELINKCVPFFLLPYVSSAVGVEVYGKIELFTTNYIILSFLFALGVEGWMNAHYFKMA ESDFITVLSSFYLLSFLIFVICMAISLPYSYYCSALLFVGYVQSLLNITTLHLRLKGKYLNAGFLLLLSS IVNAVMVYASFDWFGKTYESRINSFLFSSIIILVFIVFLSIRDKNIKIKIDFSFLNRDILLFCLPLCLTM IINWVKGNIDKYFIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKKIIFTFL FILCVFFFLYIVAVYCGFSYVFSIDYSSSRNIALLLMIGFLLLSFSSFLINFLLFYRRPRLILFQTFLIT MIHVMLSFILIKKYALLGVLLTQFVTSLLTFVSTLLVCHILTKSSQK

wzy (polymerase)

TrEMBL entry

Q077R9

Sequence

MIPYVMFYCSSLVLVVLSLVLKKYDWFWLLFLLFFSAVFVGLRVDVGADYTEYAQIYNQSGNITNFELGF DIIFNYGKRLGYDYVFVSLFFFLLTTLFFIYSIKELNYKTLIYFCFLLFMFVPLTSTIRQGLAIPFFVMC ILNSDRPKVYFTSIALGCLFHYSILFMFFFFWVRHIKQSYCRAFLIVLLFSLLSIFNIVEYIIMLLNYMP GLGAISTKLLMYTQRYQVEFSMNSFVYKLAGLLLITMFMPFIKVNEKLRCSYNLYFITVCIFILLKDNAV LTNRLTYATNISLVTFFSLLLSMIRGFYKKAIVSILFVFYFSFSYFKFTATDLRHSVESAYMPYKSLLIN

Comment

from [4]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbdN	1	1	bDGlc(1->	->3)bDGalNAc	O157 WbdN (ECODAB) O130 WffJ (ECODAB) O21 WclQ (ECODAB) O143 WfdV (ECODAB) O112ab WfeI (ECODAB) O152 WfgD (ECODAB)	3435171	[4] Also referred to as WfaM
WfaN	2	2/3	aNeu5Ac(2->	->3)bDGlc	O56 WfaR (ECODAB) O104 WbwA (ECODAB)	ABB29905	[4]
WfaO	3	2/3	aDGlc(1->	->2)bDGlc	O152 WfgE (ECODAB) O56 WfaQ (ECODAB) O29 WffY (ECODAB) O130 WffI (ECODAB) O66 WfaB (ECODAB)	ABB29908	[4]

References

[1] Carbohydr. Res. 272 (1995) 73-90 [PubMed 7544238],

[2] Eur. J. Biochem. 225 (1994) 1211-1220 [PubMed 7525286],

[3] Carbohydr. Res. 238 (1993) 335-338 [PubMed 7679327]

[4] Curr. Microbiol. 53 (2006) 470-476 [PubMed 17072668]

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Sun May 26 06:25:11 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group