Bioorganic Chemistry

Serogroup

O113

Serotype

O113:K75(B19):H21

Strain

ECODAB#

7

Structure

  ->4)aDGalNAc(1->4)aDGalA(1->3)aDGal(1->3)bDGlcNAc(1->
         |
bDGal(1->3)

CarbBank

LinucsID

Non-sugar components

acetate

Comments

Structure [1]. Position of acetate not determined

Identical to

Cross-reacts with

¹H-NMR

5.39 3.94 3.94 4.24 3.91 3.74 3.74
5.27 3.95 4.12 4.45 4.88
4.96 4.22 4.03 4.44 4.39 3.79 3.64
4.96 3.86 3.83 3.68 3.44 3.92 3.74
4.46 3.56 3.65 3.95 3.67 3.78 3.78

Conditions

Solvent: D2O; Temp.: 313K

Reference

acetone=2.230ppm

Comment

1H-NMR [1], de-O-acetylated

¹³C-NMR

100.36 67.81 76.51 66.75 71.16 61.29
97.06 68.60 69.06 79.32 71.45 172.49
99.84 49.71 78.15 76.14 70.75 60.65
102.06 55.01 81.16 71.73 75.86 61.68
105.85 71.82 73.77 69.50 75.79 62.06

Conditions

Solvent: D2O; Temp.: 313K

Reference

acetone=31.07ppm

Comment

13C-NMR [1], de-O-acetylated

wzx (flippase)

TrEMBL entry

Q9RP63

Sequence

MEKNSDKLRTGVRWSAIERIITQIAQLGILLILSRILGPKAYGLIGMLTIFIAISQTLVD SGFSSALIRRNKNSQKQYSTIFWFNLIVSLLIYAVIFFMSPHISAFYREKELSDILRVLS LLVIINALSIVHRARLTIMMDFKTQTKISLIAVLGSGVVSVILAKNGWGVWALVVNNLVF ATISTFLYFWFCKWLPDFIFQLKFFKVTAAFSVKLMIASLLNTFFDNIYQLVIGKVFYPT QVGYFTQAKNLTLLPANTYSAIIQRVTYRYFSEIKNDRVRLRQKYEEVMRISAFIFFPLT ISFSIFSNNIIVFLIGKAWEEAAKYISILSWCYVLYPIHAISLNILNVFGRSDLFLKVEI IKKIIITVCLITTYKYGISAICMGMVFYSLLATYINTIHTNRIIKTNFIALIRIISPIAF ISMLSATISFVICKFFDNNIIYIFGIMISLIIYYLIYYFVDRKIVLTIWKVNK

wzy (polymerase)

TrEMBL entry

Q9RP61

Sequence

MNTTSVICFIVFLLNPSFGCFLFLLTLFNHEKLSIFFISLFAGILAYNLVPYDTMDLASH YMKFSELHNMSVSDIWSDGVNIFLYLCMKCLNAAGINKEWLPFIATFIGYYILLNALRNN IPAYWCRKEKMICFIIWLLSISFLSLSNGIRSGLSSAFFIYAVICQRNYRHLSFIIYSVL ACMMHSFAIPMVILLFSMPLILKRKMLSQRFFKIAIIMSVSLSFLVDINSLIVFIFNNLS FLPYIDQIYNIYIIGDRWGGGASFDTNTFIARLITQAPFFYCILVLLISKRKDDFFILGA ILVSVSLFLSQFWVVSERYQYVSVLLVTLHLIFNNEYFLLRRKIIILLYFVIQTAVLIWG LYRFRYMILPSLEFIIYPFLFCFYNTVDMNKLIKV

Comment

wzx and wzy from O113:H21 strain 98NK2 [2].

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbnA	1	4	bDGal(1->	->3)aDGalNAc	O128 WbsK (ECODAB) O55:H7 WbgO (ECODAB) O167 WfdH (ECODAB) O86:K62:H2 WbnJ (ECODAB) O86:K2:H2 WcmC (ECODAB) O86:B7 WbwJ (ECODAB) O21 WclQ (ECODAB)	AAD50485	[2]
WbnB	2	2	aDGalA(1->	->3)aDGal	O55:H7 WbgM (ECODAB) O3 WclR (ECODAB) O24 WfaO (ECODAB) O22 WbhP (ECODAB)	AAD50487	[2]
WbnD	3	3	aDGalNAc(1->	->4)aDGalA	O87 WfgN (ECODAB) O22 WbhP (ECODAB) O111 WbdH (ECODAB)	AAD50489	[2]
WbnE	4	1	aDGal(1->	->3)bDGlcNAc	O3 WclR (ECODAB) O40 WffO (ECODAB) O128 WbsH (ECODAB) O55:H7 WbgM (ECODAB) O87 WfgO (ECODAB) O22 WbhP (ECODAB)	AAD50490	[2]

References

[1] Carbohydr. Res. 267 (1995) 263-269 [PubMed 7534644]

[2] Infect. Immun. 67 (1999) 5930-5937 [PubMed 10531250]

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Fri May 24 06:39:20 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group