Bioorganic Chemistry

Serogroup

O157

Serotype

O157:H7

Strain

ECODAB#

71

Structure

->2)aDRha4NAc(1->3)aLFuc(1->4)bDGlc(1->3)aDGalNAc(1->

CarbBank

LinucsID

3257

Non-sugar components

Comments

Structure from [2]. [1] gave wrong structure which was subsequently revised. Salmonella urbana and S. godesberg have the same backbone substituted with glucose on position 4 of the GalNAc residue [5].

Identical to

Citrobacter sedlakii NRCC 6070 [1], Citrobacter freundii OCU158 [1]

Cross-reacts with

Citrobacter freundii NRCC 6052 [3], Brucella melitensis [4], B. abortus [4]

¹H-NMR

4.42 3.16 3.42 3.67 3.38 3.75
4.80 3.73 3.69 3.94 3.58 1.03
4.95 4.18 3.92 4.12 4.07 1.00
5.09 3.79 3.9  3.87      3.59

Conditions

Solvent: D2O; Temp.: 70C

Reference

acetone=2.225ppm

Comment

1H-NMR [1]

¹³C-NMR

103.4 73.0 73.9 77.8 74.7 59.5
 98.9 66.7 71.4 76.6      16.4
 99.5 66.6 70.8 48.5 68.2 14.7
100.4 52.8 67.8 77.7      60.9

Conditions

Solvent: D2O; Temp.: 70C

Reference

acetone=31.07ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

O85337

Sequence

MIMNKIKKILKFCTLKKYDTSSALGREQERYRIISLSVISSLISKILSLLSLILTVSLTL PYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLA GLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLGIYSNGVQKVYMG IQKAYISNIVNAIFILLSIITLVISSKLHAGLPVLIVSTLGIQYISGIYLTINLIIKRLI KFTKVNIHAKREAPYLILNGFFFFILQLGTLATWSGDNFIISITLGVTYVAVFSITQRLF QISTVPLTIYNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVV NIWTEGKIQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKY IIVSHFGLTVMLYCFIFIYIVNYFIWYKCSFKKHIDRQLNIRG

wzy (polymerase)

TrEMBL entry

O85335

Sequence

MKSAAKLIFLFLFTLYSLQLYGVIIDDRITNFDTKVLTSIIIIFQIFFVLLFYLTIINER KQQKKFIVNWELKLILVFLFVTIEIAAVVLFLKEGIPIFDDDPGGAKLRIAEGNGLYIRY IKYFGNIVVFALIILYDEHKFKQRTIIFVYFTTIALFGYRSELVLLILQYILITNILSKD NRNPKIKRIIGYFLLVGVVCSLFYLSLGQDGEQNDSYNNMLRIINRLTIEQVEGVPYVVS ESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGERLHKQVFGDMGANFLSVTTYGAEL LVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSINCAFYSYIIMILLQYLVAGNASAFFFG PFLSVLIMCTPLILLHDTLKRLSRNENISYNCDL

Comment

wzx and wzy are from isolate C664-1992 (Statens Serum Institut, Copenhagen, Denmark) [6]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbdN	1	1	bDGlc(1->	->3)aDGalNAc	O24 WbdN (ECODAB) O130 WffJ (ECODAB) O21 WclQ (ECODAB) O143 WfdV (ECODAB) O112ab WfeI (ECODAB) O152 WfgD (ECODAB) O91 WbsA (ECODAB) O79 WbdX (ECODAB)	AAC32339	[6]
WbdO	2				O168 WfeV (ECODAB) O168 WfeY (ECODAB) O159 WfgS (ECODAB) O159 WfgP (ECODAB)	AAC32341	[6]
WbdP	3					AAC32344	[6]

References

[1] FEMS Immunol. Med. Microbiol. 28 (2000) 163-171 [PubMed 10799808], revised in FEMS Immunol. Med. Microbiol. 32 (2002) 255.

[2] Biochem. Cell Biol. 64 (1986) 21-28 [PubMed 3008786]

[3] FEMS Microbiol. Lett. 190 (2000) 157-161 [PubMed 10981707]

[4] Infect. Immun. 58 (1990) 1391-1395 [PubMed 1691146]

[5] Carbohydr. Res. 156 (1986) 107-122 [PubMed 3815404]

[6] Infect. Immun. 66 (1998) 3545-3551 [PubMed 9673232]

Edit this entry

Sun May 19 03:37:51 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group