Bioorganic Chemistry

Serogroup

O66

Serotype

O66:K-:H25

Strain

ECODAB#

77

Structure

->2)bDGlc3Ac(1->3)aL6dTal(1->3)aDGlcNAc(1->2)bDMan(1->3)aDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

acetates

Comments

Structure [1]

Identical to

Cross-reacts with

Escherichia coli 4610 (O45) and 3332 (O45rel) [1]

¹H-NMR

5.02 3.89 3.87 3.64 4.24 1.21
5.11 3.98 3.88 3.56 4.02 3.82 3.82
4.73 3.92 3.76 3.71 3.40 3.94 3.80
5.43 4.06 3.95 3.56 4.10 3.80 3.80
4.74 3.52 3.59 3.47 3.43 3.77 3.72

Conditions

Solvent: D2O; Temp.: 90C

Reference

acetone=2.225ppm

Comment

1H-NMR [1]

¹³C-NMR

102.8 70.9 76.5 71.7 68.9 16.7
99.6 55.0 81.0 69.3 73.1 61.7
101.4 78.1 74.9 68.2 78.1 62.0
97.2 53.9 81.3 69.3 73.1 61.6
104.8 75.7 77.4 67.0 76.8 61.6
23.3 174.7 23.3 174.4 21.45 173.9

Conditions

Solvent: D2O; Temp.: 90C

Reference

acetone=31.45ppm

Comment

13C-NMR [1]

wzx (flippase)

TrEMBL entry

Q1AL85

Sequence

MNYLVNRKDIIWGYLSQFLNISSSLLLLPFILKCLNEEEIGLWYVFVAMAGIIQLLEFGLLPTISRFISY VYSGALNLEYGKIPKCNIGDFNKELLNDVVVTCRCIYFKIALFALVGILILGNAYIYTLSTSINKYYILC CWSVYGLSICIQLYFGYYNAILKGRGDQTLLNKIIVVAKLTLLLISIPLLIAGCGLVSLAVGAVISVIID RILIRKYVYSQESGLIRNLEKSNKTVSLKKVIWQSARDMGVVQLGNYLSVRAGVLIVSSFVNLDAAASYG LTVQVTMVIVIIASMFFGLNIPRITSEQAHENMLVVKNLMKKSLLIANCIYIFAALILVAIGDDILAFLT KNTTLLPKELLILYLVTALFEMNYSICTSYLTTRNEVIFIKSMLYTGVAIVLFSFISTYIAEMGALGVIL SQLLMQSLYNNWHWPLKVYQELKND

wzy (polymerase)

TrEMBL entry

Q1AL83

Sequence

MKNKISFYIAYIYIAPIILSLYYISTTNSLLGDFRTYSYKLEWESSLLFLILYFIPLIIIFCVVKMTGKI KVDTFESIKLEKFAFYSIIITGLITLIFGANAIGHPAQTGIAGVIIKIASKLNPLVLLPLLTFTNVSVRK FFFCAAVVIFYGYMQQSLQGIYISVLCAGAFFLLKVRLNGAIFILMLLSPIIFLGPLLDIITHLYTIRNE IRGVSFSVEEIFSLALGRISTTSSLIYTISNSFNTTGVSDYFVFGIVIERLIGVHILDTVSPSQVFNTYI LGANADYSIFMGLAGFLYFIKDFTCSVYCKCCVFDLGTDSYILGANADYSIFMGLAGFFVFLLKTSPAVF IVNAACLILVLILIYLLIPVNNVKYRTSVFFLIMYLPFLSFDIWEISIVFQTIIIWRVFLLLSKISFGLK

Comment

From [2]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WfaA	1					AAZ20760	[2]
WfaB	2	2	aDGlcNAc(1->	->2)bDMan	O152 WfgE (ECODAB) O56 WfaQ (ECODAB) O29 WffY (ECODAB) O24 WfaO (ECODAB)	AAZ20762	[2]
WfaC	3	4	bDGlc(1->	->3)aL6dTal	O55:H6 WbgP(ECODAB) O55:H7 WbgP(ECODAB) O112ab WfeF (ECODAB) O24 WbdN (ECODAB) O113 WbnA (ECODAB) O143 WfdV (ECODAB)	AAZ20763	[2]
WfaE	4					AAZ20765	[2]

References

[1] Carbohydr. Res. 278 (1995) 155-165 [PubMed 8536267]

[2] J. Microbiol. 45 (1) (2007)69-74 [PubMed 17342059]

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Fri May 24 20:00:02 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group