Bioorganic Chemistry

Serogroup

O28

Serotype

O28ac:H-

Strain

ECODAB#

8

Structure

->2)(R)Gro-1-P(O->4)bDGlcNAc(1->3)bDGalf2Ac(1->3)aDGlcNAc(1->

CarbBank

LinucsID

Non-sugar components

acetate, glycerol, phosphate

Comments

Structure [1]

Identical to

Cross-reacts with

¹H-NMR

4.69 3.72 3.76 3.97 3.51 3.78 3.89 2.08
5.21 4.88 4.35 4.26 3.92 3.64 3.68
5.01 4.06 3.86 3.47 3.84 3.80 3.86 2.05
4.02 4.06 3.89 3.76 3.76

Conditions

Solvent: D2O; Temp.: 50C

Reference

acetone=2.225ppm

Comment

1H-NMR [1], pD=6

¹³C-NMR

101.1 56.1 73.6(JCP=2.4) 75.0(JCP=6.2)
75.9(JCP=5.5) 61.3 23.2 175.3
106.7 82.4 83.3 83.2 71.0 63.8 21.0 173.3
97.8 53.9 77.9 69.1 73.1 61.4 23.0 174.8
65.2(JCP=5.8) 78.1(JCP=7.4) 62.1

Conditions

Solvent: D2O; Temp.: 50C

Reference

acetone=31.00ppm

Comment

13C-NMR [1], pD=6

wzx (flippase)

TrEMBL entry

A4K7Z5

Sequence

MSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIFLCVIFLYFLVNDQLQQFKIEFFFYSC IAIIGQTFQSLWFFQGVEKMKRITLFLTISKFSYVILTFVIVKKPEQGRFVILAYALSQVIGALLSQYYM RKEGYKYTPANLSSMLGVLRTSALFFWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTP VSQALYPYMTRAKNWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLALIVTF VSTNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLFTEIVILILRMFFFVKL YRREVK

wzy (polymerase)

TrEMBL entry

A4K7Z7

Sequence

MKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAYVLFAISLVAVYYAQGESYTSLGFLFN TCLFLFIGGRFIATTFGIDSESVFTLDFMTTYIPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLN KNDNSLPGRLLFLIVIVLFLLCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFAT CKKKYIIINFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIMNFSA RGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFASMFHGGSDLNSYMLSFP AYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSVLIGMFLKFANEQAKRNSFWFGAVACLCA KILFTARADIKTIIPLFMYYAIMYLIISTAMRILRQRYG

Comment

Deposited as DQ462205 in Genbank [2] (Unpublished).

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbeS	1	3	(R)Gro-1-P(O->	->4)bDGlcNAc		ABF01009	glycerol phosphotransferase [2]
WbeX	2	2	bDGlcNAc(1->	->3)bDGalf	O42 WbeX (ECODAB) O112ab WfeF (ECODAB)	ABF01011	[2]
WbeY	3	1	bDGalf(1->	->3)aDGlcNAc	O42 WbeY (ECODAB) 167 WfdK (ECODAB) 167 WfdJ (ECODAB)	ABF01012	[2]

References

[1] Carbohydr Res. (1996) 127-39 [PubMed 8864227]

[2] Sequencing of the Escherichia coli O2, O28ac, O63 and O42 O antigen clusters and detection of pathogenic strains by multiplex PCR assays targeting virulence genes in the respective O-antigen gene clusters. Submitted (10-DEC-2008).

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Sun May 19 03:15:41 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group