Bioorganic Chemistry

Serogroup

O55

Serotype

O55:H7

Strain

ECODAB#

80

Structure

            ->6)bDGlcNAc(1->3)aDGal(1->3)bDGalNAc(1->
                   |
aCol(1->2)bDGal(1->3)

CarbBank

LinucsID

Non-sugar components

Comments

Structure [1]

Identical to

Salmonella greenside group Z [2]

Cross-reacts with

¹H-NMR

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

¹³C-NMR

100.6 34.9 17.4 102.4 103.3 56.6 97.1 103.7 52.4

Conditions

Solvent: D2O; Temp.:

Reference

not stated=ppm

Comment

13C-NMR [1]. External TMS

wzx (flippase)

TrEMBL entry

Q8VQ44

Sequence

MIKKMFSSGFIKNGISNYICVAWMGGVSIIVLPYYLHILGPQQWGIVAICISIQSLMYLA DAGMSQIMPRDIARCNGKSRELYVIYKSFLHAYSLLAIIVFSLILVGSYFIIDFWLVLDF SLKKEVFICVVLLGLQFLFQFPNNANIGYWNGTLQQHKSNIRQCFFFTLKHIVAICLVTK WPQAILYQSGFLLIAFLESTSNTIKILFSFDGNIKRQEKLSIGINKIFRESGVLSIAVAI GLLVTQLDRIVLSKYVDISDFGYYVLASTLAQYFLQLQYPLLRLFFPRLTVALNKQNILR LLYSIIIFCVFPCLIMVLISPFFLSLWLDNAEAESIIIWPLRLILIAIAINSIYNIIYQI MLIKQKAIVILCINISSLLCVFPTIWLLSTRVGVLAGGIGWVLCSLIQLFISLLWLFKRK ESILL

wzy (polymerase)

TrEMBL entry

Q8VQ45

Sequence

MFNKKYVVIIWGGVLSLLCSLPLSIQGGFDDSLVYYEQAIYIWNNGLLEGLSSIYHQTNK FEIGMGLFFYLQGFLGEGRFVFILLNLFLVNFLAVLIYLKINDEVRISPSLLCVTLMLMS YYVFSNNIYVWRSIICLYFLVLMIYSKTKYKKIIFIGLGLLFHYTFILFLCVYYFCRVNK IGKFKFVIVSIIISFIISNFLYWMSYASLFVSGGELSLFMSQDNEALKRLIISGTFLFLL LMINLESETNNWVLYKLSLFFCILSIFLCDNWQLSWRVFVPAAILGTAIILSNIKKKDSV VLLGITLSIIPTFRLIFNLLILGHP

Comment

wzx and wzy from O55:H7 strain M1685, P. Tarr, U. Washington [3].

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbgM	1	1	aDGal(1->	->3)bDGalNAc	O128 WbsH (ECODAB) O113 WbnE (ECODAB) O3 WclR (ECODAB) O40 WffO (ECODAB) O87 WfgO (ECODAB) O22 WbhP (ECODAB) O138 WfaH (ECODAB)	168986365	[3]
WbgN	2	4	aCol(1->	->2)bDGal	O126 WbgL (ECODAB) O128 WbsJ (ECODAB) O127 WbiQ (ECODAB) O86:B7 WbwK (ECODAB) O86:K62:H2 WbnK (ECODAB)	AAL67556	[3]
WbgO	3	3	bDGal(1->	->3)bDGlcNAc	O126 WbsT (ECODAB) O127 WbiP (ECODAB) O86:B7 WbnJ (ECODAB) O86:K2:H2 WcmC (ECODAB) O86:B7 WbwJ (ECODAB) O128 WbsK (ECODAB) O113 WbnA (ECODAB) O167 WbsK (ECODAB)	AAL67559	[3]
WbgP	4	2	bDGlcNAc(1->	->3)aDGal	O55:H6 WbgP (ECODAB) O157 WbdN (ECODAB) O24 WbdN (ECODAB) O66 WfaC (ECODAB) O152 WfgD (ECODAB)	AAL67560	[3]

References

[1] Carbohydr. Res. 97 (1981) 105-112 [PubMed 7030487]

[2] Carbohydr. Res. 111 (1983) 289-296 [PubMed 6339053]

[3] J. Bacteriol. 184 (2002) 2620-2625 [PubMed 11976290]

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Fri May 24 22:32:14 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group