Bioorganic Chemistry

Serogroup

O111

Serotype

Strain

ECODAB#

88

Structure

aCol(1->6)
        |
 ->4)aDGlc(1->4)aDGal(1->3)bDGlcNAc(1->
        |
aCol(1->3)

CarbBank

LinucsID

4786

Non-sugar components

Comments

Structure [1]. Very poor NMR data - caution

Identical to

Salmonella adelaide, group O [2] - but see also [3]

Cross-reacts with

¹H-NMR

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

¹³C-NMR

102 101 82 79 78 77 75 74.5 73 72 70.5 69
68 65 63 61.5 56 35 34.5 24 17.4 17.1

Conditions

Solvent: not stated; Temp.:

Reference

not stated=ppm

Comment

13C-NMR [4]. Manually extracted from picture. Shifts +/-=0.5 ppm! Some signals very broad, some arise from several overlapping resonance. A signal at 31.0 ppm is possibly acetone.

wzx (flippase)

TrEMBL entry

Q46722

Sequence

MVLTVKKILAFGYSKVLPPVIEQFVNPICIFIITPLILNHLGKQSYGNWILLITIVSFSQ LICGGCSAWIAKIIAEQRILSDLSKKNALRQISYNFSIVIIAFAVLISFLILSICFFDVA RNNSSFLFAIIICGFFQEVDNLFSGALKGFEKFNVSCFFEVITRVLWASIVIYGIYGNAL LYFTCLAFTIKGMLKYILVCLNITGCFINPNFNRVGIVNLLNESKWMFLQLTGGVSLSLF DRLVIPLILSVSKLASYVPCLQLAQLMFTLSASANQILLPMFARMKASNTFPSNCFFKIL LVSLISVLPCLALFFFGRDILSIWINPTFATENYKLMQILAISYILLSMMTSFHFLLLGI GKSKLVANLNLVAGLALAASTLIAAHYGLYAISMVKIIYPAFQFYYLYVAFVYFNRAKNV

wzy (polymerase)

TrEMBL entry

Q9S521

Sequence

MSIDLLFSITEIAIVFSCTIYIFTQCLLMRRIYLDKSILILLCLLFFLVIIQLPELNVNG LVDSLKLSLPLLMVFIAFQKPKLCLWVIIALLFLNSAFNFLYLKTFDKFSSFPFTFFILL FYLFRLGIGNLPVYKNKKFYALIFLFILIDIMQSLLINYRGQILYSVICILILVFKVNLR KKIPYFFLMLPVLYVIIMAYIGFNYFNKGVTFFEPTASNIERTGMIYYLVSQLGDYIFHG MGTLNFLNNGGQYKTLYGLPSLIPNDPHDFLLRFFISIGVIGALVYHSIFFVFFRRISFL LYERNAPFIVVSCLLLLQVVLIYTLNPFDAFNRLICGLTVGVVYGFAKIR

Comment

From O111 strain Stoke W [5]

GT name	Position in gene sequence	Linkage number	Donor	Acceptor	Similar to	BLAST	Comments
WbdH	1	1	aDGal(1->	->3)bDGlcNAc	O121 WbqI (ECODAB) O138 WfaH (ECODAB) O52 Orf16 (ECODAB) O113 WbnE (ECODAB)	5678715	[5], [6]
WbdL	2	2	aDGlc(1->	->4)aDGal	O168 WfeY (ECODAB) O159 WfgS (ECODAB) O168 WfeV (ECODAB)	AAD46731	[5], [6]
WbdM	3	3, 4	aCol(1->	->3)aDGlc ->6)aDGlc	O121 WbqH (ECODAB) O87 WfgO (ECODAB) O113 WbnE (ECODAB)	AAD46732	[5], [6]

References

[1] K. Eklund, P.J. Garegg, L. Kenne, A.A. Lindberg, B. Lindberg (1978). Structural studies on the Escherichia coli O111 lipopolysaccharide. Abstr 493, In Abstracts of the IXth International Symposium of Carbohydrate Chemistry, London, 1978

[2] Carbohydr. Res. 111 (1983) 289-296 [PubMed 6339053]

[3] J. Biol. Chem. 242 (1967) 4125-4133 [PubMed 4863044]

[4] Infect Immun. 63 (1995) 2805-2810 [PubMed 7542631]

[5] J. Clin. Microbiol. 36 (1998) 3182-3187 [PubMed 9774562]

[6] Appl Environ Microbiol. 74(4)(2008) [PubMed 18156323]

Edit this entry

Sat May 18 17:13:06 CEST 2013

Bioorganic Chemistry

The Widmalm Research Group