O-Antigen Description

Serogroup:O58
Serotype H:27
Serotype K:
Strain:
Structure:
          ->4)bDMan(1->4)aDMan(1->3)bDGlcNAc(1->
                 |
aLRha2Ac3RLac(1->3)
Comment:- Structure [3]
- Rha3Rlac2Ac=2-O-acetyl-3-O-[(R)-1-carboxyethyl]-L-rhamnose
- Structure is identical to S. dysenteriae type 5
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WffC
GT nameDonorAcceptorPosition | Link.NumSimilar to
WffD

bDMan(1->

->4)aDMan

GT NameDonorAcceptorScore / e-value
O78-WejN

bDMan(1->

->4)aDMan

299 / 2e-100
GT nameDonorAcceptorPosition | Link.NumSimilar to
WffE

aDMan(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O88-O088_13


486 / 0.0
O78-WejO


395 / 4e-138
O115-WegL

aDMan(1->

->3)bDGlcNAc

313 / 4e-106
O141-WfcD


305 / 5e-103
O187-O187_13


301 / 7e-102
O144-O144_04


300 / 2e-101
O34-O034_12


252 / 1e-82
O99-WejK

DRha(1->

->?)?DGlcNAc ->?)?DGalNAc

185 / 2e-56
O60-O060_12


153 / 1e-44
O169-O169_07


151 / 6e-44
O183-wbaY


142 / 7e-41
O175-O175_06


135 / 3e-38
O9-WbdC

aDMan(1->

->2)aDMan ->3)aDMan

133 / 7e-38
NMR
1H-NMR4.93 4.12 3.67 3.57 4.28 1.29 4.68 4.19 3.79 3.96 3.45 3.73 3.90 5.24 4.05 3.89 3.90 3.64 3.77 3.81 4.50 3.74 3.68 3.42 3.40 3.60 3.88 4.28 1.45
Solvent: D2O
Temp: 40C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [3]
13C-NMR95.7 70.7 78.9 72.7 70.0 18.1 101.4 68.3 77.1 71.4 77.1 61.7 102.1 71.3 70.5 77.5 73.0 61.7 101.6 56.0 81.1 73.0 77.5 63.2 181.0 76.2 20.1
Solvent: D2O
Temp: 40C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [3]
Non-Sugar Components
- Acetate
Enzymes
FlippaseMKINACLSFGTTLCKMITGLLFLAILSHSINNAEYSIIVTSIFFAQLLSVFVDGGINNEVLSLTNKSLDN DIDGYQKLSMNGAVRLVVHLFFSFVLSTYFMFADGWSAGGVFFLGYISGVLTSILETYSIKLKTEYKYIE DFILNIFCSLFVILSSFFIYIFPQCLMLYLVFFRLIPLAIYRKPLQVFKMIREIDYKLIRDNYIARKHYS IDSIATNINLQLDSFFLLLLFGRDMYAYYQPLNRLYNSCIGLSAAVVSFAIPYAHLLTSRIKKIYFLVFL FSSSAIIISLSYYFFSRDVVMVFFGEKFSMERQYIFLFSLLIFMRYLCASFGSYLMINGEQKKRALINLT ITILCIPFILISETYSGILMVIIGGQILISYFYVLMIKRGVGHDK
Trembl: B5L3K7
PolymeraseMYANKAMPKRHTRERVFLPLLLSMIFICMIINDLPLQQYLGTLGASPMWGGALFIFMLIVIRSRFVLNLD STSKYFLYFYLLTFTISLLQCFYYAISRGSVENEYGGLIFGKLVFASTYYIVYFFTIYTAIFLARRITSS ALKFCIINASVLLLLLLILEFFSPNSLSLFHKSMDGYGFGFRQRLLSPEPSMAAFTLNIFLLISIVLVNS KIAKLFMGGALVVGNLFIGSKASLILILMSGVIVFYLNMNLTQKIKSLLILIPVSIAVFYVFLNTILPAL IVDVDKFTSVSTRLITALAALLSLIYYPLGEGYGTYSSYLLGPLDLAVQLAEELLPFTLNVSEVNKMLMT GESLAAKSGILFSVIQSGVVSLIFFYVIYRNAFRKLCAARLSNYNKIMMRLVLWYSLLSIMFAVNIEVVY AFLLPFIIIDHYAINYMRSYDYE
Trembl: B5L3L2
Literature
  • First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
  • First Author: Dmitriev, BA. "Cell-wall lipopolysaccharide of the "Shigella-like" Escherichia coli 058. Structure of the polysaccharide chain". European Journal of Biochemistry. 79(1). (1977), pp 111-5 PubMed: [334542]
  • First Author: Perepelov AV. "Antigenic Polysaccharides of Bacteria: 41. Structures of the O-Specific Polysaccharides of Shigella dysenteriae Types 4 and 5 Revised by NMR Spectroscopy". Bioorg. Khim. 34(4). (2008), pp 513-521 PubMed: [18695724]